mProX™ Human LTB4R2 Stable Cell Line

Product Information

Target Protein

LTB4R2

Target Family

Leukotriene Family

Target Protein Species

Human

Host Cell Type

Huh7;CHO-K1;HEK293

Target Classification

GPCR Cell Lines

Target Research Area

Immunology Research;Autoimmune Research

Related Diseases

Psoriasis 5;Persistent Mild Asthma

Gene ID

Human: 56413

UniProt ID

Human: Q9NPC1

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

LTB4R2 has been identified as a significant player in various diseases, particularly in the context of cancer. A functional genomics analysis on lung cancer histotypes identified LTB4R2 as a relevant druggable essentiality gene. Furthermore, BLT2 expression, another name for LTB4R2, has been found to potentially contribute to the regulation of Tumor-Associated Macrophages (TAMs), T cell exhaustion, and Tregs activation in clear cell Renal Cell Carcinoma (ccRCC).

Protocols

Please visit our protocols page.

Customer Reviews

There are currently no Customer reviews or questions for mProX™ Human LTB4R2 Stable Cell Line (S01YF-0923-PY93). Click the button above to contact us or submit your feedback about this product.

FAQ

chat Sandra (Verified Customer)

What are the recent advancements in the study of LTB4R2 in the context of membrane proteins? Jul 23 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

Although there is limited direct information on LTB4R2, advancements in membrane protein research, such as the development of new tools and techniques for structural studies of eukaryotic membrane proteins, could potentially be applied to study LTB4R2. Jul 23 2022

chat Lisa (Verified Customer)

What potential applications does LTB4R2 have in biomedical research? Apr 27 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

While specific information on LTB4R2 is limited, studying this receptor within the broader context of membrane proteins could contribute to understanding cellular processes, disease mechanisms, and the development of new therapeutic targets. Apr 27 2021

Published Data

Fig.1 12-HHTrE-LTB4R2 signaling activates YAP1 .

Utilizing immunofluorescence microscopy, we assessed nYAP1 levels in Huh7 cells cultured at approximately 40% density and infected with adenovirus expressing EGFP alongside LTB4R2-shRNA or SCR-shRNA. Morphometric analysis yielded the proportion of nuclear YAP1+ cells. A non-infected control (NT) was included. Results are expressed as means ± SEM from three independent experiments. Significance (**p < 0.01) was determined via a two-sided t-test, comparing against SCR-shRNA cells.

Ref: Sinha, Sonal, et al. "Hepatic stellate cell stearoyl co-A desaturase activates leukotriene B4 receptor 2-β-catenin cascade to promote liver tumorigenesis." Nature communications 14.1 (2023): 2651.

Pubmed: 37156770

DOI: 10.1038/s41467-023-38406-8

Research Highlights

Hashemi Karoii D, et al. "Altered G-Protein Transduction Protein Gene Expression in the Testis of Infertile ." DNA and cell biology, 2023.
Recent studies have shown that members of the G-protein-coupled receptors (GPCR) superfamily play key roles in sperm and Sertoli cell function. A microarray was used to analyze GPCR, guanyl-nucleotide exchange factor, membrane traffic protein, and small GTPase gene expression in human cases with nonobstructive azoospermia. Results revealed upregulation of GOLGA8IP, OR2AT4, PHKA1, and others, while MARS, SIRPG, OGFR, and others were downregulated. Sertoli cells of nonobstructive azoospermia cases also showed changes in gene expression. Further analysis of molecular interactions and master pathways was conducted. The enrichment analysis showed significantly expressed biological processes and molecular functions in both sperm and Sertoli cells. These findings can help develop receptor-selective GPCR agonists or antagonists in the future.
Pubmed: 37610843 DOI: 10.1089/dna.2023.0189

Yang Y, et al. "Inhibition of neuroactive ligand-receptor interaction pathway can enhance ." Expert review of anticancer therapy, 2023.
The association between Homologous recombination deficiency (HRD) and immunotherapy in colon cancer has not been fully explored. In this study, the authors gathered exon sequencing and transcriptome data from 456 colon adenocarcinoma (COAD) patients in the TCGA database. Through GSVA methods, they calculated the pathway activity score and performed survival analysis. The prognostic value of these pathways was confirmed in an external GEO cohort and an immunotherapy cohort. Results showed that high HRD levels were correlated with worse prognosis, lower tumor mutation burden and microsatellite instability, and decreased sensitivity to immunotherapy in COAD. Additionally, the neuroactive ligand-receptor interaction pathway was found to be over-activated in high-HRD tumors and associated with immune suppression in colon cancer. This pathway was also linked to prognosis and immunotherapy response in COAD. Furthermore, the authors identified LTB4R2 as a potential target for the development of therapies for colon cancer. In conclusion, this research highlights the potential mechanism of HRD and sheds light on the role of the neuroactive ligand-receptor interaction pathway in colon cancer, providing insights for improving immunotherapy response in this type of cancer.
Pubmed: 37555253 DOI: 10.1080/14737140.2023.2245567