mProX™ Human LTB4R Stable Cell Line

Lundgren SM, et al. "Signaling dynamics distinguish high- and low-priority neutrophil chemoattractant ." Science signaling, 2023.
Neutrophils, a type of white blood cell, possess the ability to migrate from blood vessels to sites of infection and injury in response to various signals. These signals may include those produced by pathogens or injuries, as well as long-range inflammatory signals, like LTB4. Despite activating the same G protein family, different chemoattractants can induce unique modes of migration, with high-priority signals causing sustained responses and low-priority signals resulting in transient responses. Through live-cell imaging, it was observed that this difference in signaling dynamics is due to temporal regulation of shared signaling pathways. Specifically, the rapid attenuation of Cdc42 activation in response to LTB4 is dependent on specific phosphorylation sites on the LTB4 receptor, LTB4R, and is not affected by endocytosis. Mutating these sites impairs the ability of neutrophils to prioritize chemoattractants, but does not affect the persistence of migration. Overall, these findings demonstrate the importance of distinct temporal regulation in differentiating between chemoattractants and highlight the role of Ca(2+) and Cdc42 in regulating neutrophil responses.
Pubmed: 37788324   DOI: 10.1126/scisignal.add1845

McFarlin BK, et al. "Dry blood spot samples to monitor immune-associated mRNA expression in ." Methods (San Diego, Calif.), 2023.
In this study, the authors aimed to investigate the feasibility of utilizing their optimized dry blood spot (DBS) methodology to monitor the immunological response to physical stressors in a field setting. This was in contrast to traditional methods which require a laboratory visit and venipuncture for blood collection. The study focused on the effect of Baker's Yeast Beta Glucan supplementation (BYBG; Wellmune; 250 mg/d) on post-exercise mRNA expression. Participants underwent a 90-minute run/walk trial in a hot, humid environment and their venous DBS samples were collected at various time points. Following total RNA extraction and analysis using a 574-plex Human Immunology mRNA panel (Nanostring), the researchers observed that BYBG supplementation was associated with significant changes in the expression of several mRNAs and immune-response pathways. These changes indicated that BYBG may support the immune system, reduce susceptibility to opportunistic infection and improve physical recovery from exercise. The authors conclude that their DBS methodology can be used to investigate the effects of other nutritional, health, or medical interventions in the future.
Pubmed: 37741562   DOI: 10.1016/j.ymeth.2023.09.006