mProX™ Human LRRK2 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Assay Types
S01YF-1122-KX1193	Magic™ Human LRRK2[I2020T] in Vitro Assay	Human	Kinase Assay
S01YF-1122-KX1194	Magic™ Human LRRK2[G2019S] in Vitro Assay	Human	Kinase Assay
S01YF-1122-KX1195	Magic™ Human LRRK2[R1441C] in Vitro Assay	Human	Kinase Assay

Product Information

Target Protein

LRRK2

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

CNS Research; Reproductive Research

Related Diseases

Parkinson Disease 8, Autosomal Dominant and Hereditary Late-Onset Parkinson Disease. Among its related pathways are Signaling by PTK6 and ncRNAs involved in Wnt signaling in hepatocellular carcinoma

Gene ID

120892

UniProt ID

Q5S007

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human LRRK2 gene encodes leucine-rich repeat kinase 2 (LRRK2), a big, multifunctional kinase enzyme also referred to as dardarin and PARK8. LRRK2 belongs to the family of leucine-rich repeat kinases. Variants in this gene have been linked to a higher incidence of Crohn's and Parkinson's disease. The WD40 domain, kinase domain, RAS domain, GTPase domain, leucine-rich repeat (LRR) domain, ankyrin repeat (ANK) region, and armadillo repeats (ARM) domain are all present in the protein that is encoded by the LRRK2 gene. The protein connects with the outer membrane of the mitochondria but is mostly found in the cytoplasm. The customized LRRK2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Brian

This LRRK2 overexpression cell line is sensitive and specific. I definitely recommend this cell line. Jul 29 2020

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chat Timothy

The LRRK2 cell line was easy to use and provided excellent results in my experiments. Apr 25 2023

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 LRRK2 is expressed in immune cells.

In order to verify the existence of the distinct amplicons belonging to LRRK2 and GAPDH, reaction products were put into acrylamide gels (left panel). On the right is a quantification of the ratio of LRRK2 mRNA to GAPDH mRNA, normalized to the value measured in THP-1 cells.

Ref: Gardet, Agnès, et al. "LRRK2 is involved in the IFN-γ response and host response to pathogens." The Journal of Immunology 185.9 (2010): 5577-5585.

Pubmed: 20921534

DOI: 10.4049/jimmunol.1000548

Research Highlights

As a result of this work, LRRK2 has been implicated in a confusing array of cell biological processes and pathways and may play a role in several seemingly unrelated disease problems.
Berwick, Daniel C., et al. "LRRK2 Biology from structure to dysfunction: research progresses, but the themes remain the same." Molecular neurodegeneration 14.1 (2019): 1-22.
Pubmed: 31864390 DOI: 10.1186/s13024-019-0344-2

Leucine-rich repeat kinase 2 (LRRK2) missense mutations result in familial Parkinson's disease (PD). Uncertainty surrounds the possible involvement of wild-type LRRK2 in idiopathic PD (iPD).
Di Maio, Roberto, et al. "LRRK2 activation in idiopathic Parkinson's disease." Science translational medicine 10.451 (2018): eaar5429.
Pubmed: 30045977 DOI: 10.1126/scitranslmed.aar5429