mProX™ Human LPAR2 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- GPCR Cell Lines
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Published Data
Fig.1 LPAR2 knockdown reduced LPA-induced proliferation.
AGS (on the left) and NCI-N87 (on the right) cell lines underwent lentiviral transduction with either a control vector or three distinct LPAR2-shRNA constructs. Subsequently, their response to LPA-induced proliferation was assessed through hemocytometry.
Ref: Ara, Hosne, et al. "Alteration of Cellular Energy Metabolism through LPAR2-Axin2 Axis in Gastric Cancer." Biomolecules 12.12 (2022): 1805.
Pubmed: 36551233
DOI: 10.3390/biom12121805
Research Highlights
Takai M, et al. "Induction of lysophosphatidic acid (LPA) receptor-mediated signaling regulates ." Advances in biological regulation, 2023.
In this study, the researchers evaluated the effects of LPA receptors on the motility and survival of colon cancer DLD-1 cells in the presence of hydrogen peroxide (H(2)O(2)). Lysophosphatidic acid (LPA) signaling through LPA receptors (LPA(1)-LPA(6)) was found to regulate various cellular functions such as growth, migration, and differentiation. To obtain H(2)O(2)-treated DLD-1 cells (DLD-H(2)O(2)), the cells were cultured in a medium containing 60 µM H(2)O(2) for two months. LPAR2 and LPAR4 gene expression levels were significantly increased in DLD-H(2)O(2) cells. The motility of DLD-H(2)O(2) cells was lower compared to DLD-1 cells and was suppressed by LPA(2) knockdown but stimulated by LPA(4) knockdown. DLD-H(2)O(2) cells also exhibited a higher survival rate to anticancer drugs, fluorouracil (5-FU), irinotecan (CPT-11), and oxaliplatin (L-OHP), compared to DLD-1 cells. Knockdown of LPA(2) reduced the survival rate to 5-FU in DLD-H(2)O(2) cells, while knockdown of LPA(4) increased the survival rate. In a hypoxic environment, DLD-H(2)O(2) cells cultured at 1% oxygen showed a higher survival rate to 5-FU compared to DLD-1 cells, which correlated with LPAR2 gene expression. These findings suggest that LPA receptor-mediated signaling is important in regulating cellular functions in H(2)O(2)-treated DLD-1 cells, particularly in the tumor microenvironment with high H(2)O(2) levels.
Pubmed:
37603941
DOI:
10.1016/j.jbior.2023.100978
Okuda A, et al. "Roles of lysophosphatidic acid (LPA) receptor-2 (LPA(2)) in the regulation of ." International journal of radiation biology, 2023.
The roles of Lysophosphatidic acid (LPA) receptor-2 (LPA(2)) in cellular responses to X-ray irradiation and hydrogen peroxide (H(2)O(2)) treatment were studied in pancreatic cancer PANC-1 cells. LPAR2 expression levels were analyzed, and the effects of LPA(2) knockdown on cell survival and motility were evaluated. X-ray irradiation increased LPAR2 expression and decreased cell motility in PANC-1 cells, with enhanced effects in LPA(2) knockdown cells. GRI-977143 (LPA(2) agonist) increased cell survival to X-ray irradiation and CDDP treatment in H(2)O(2) treated cells, while LPA(2) knockdown had the opposite effect. These findings demonstrate the crucial role of LPA(2)-mediated signaling in the regulation of cellular functions in PANC-1 cells following exposure to X-ray irradiation and H(2)O(2).
Pubmed:
37523658
DOI:
10.1080/09553002.2023.2241890
