mProX™ Human LIMK1 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1191	Magic™ Human LIMK1 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

LIMK1

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Reproductive Research; Cardiovascular Research

Related Diseases

Williams-Beuren Syndrome and Supravalvular Aortic Stenosis. Among its related pathways are Semaphorin interactions and Signaling by Rho GTPases

Gene ID

3984

UniProt ID

P53667

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Instructions for producing a highly active protein in the brain, where it is believed to be crucial in nerve cell growth and development, are provided by the LIMK1 gene. Research indicates that this particular protein could have a significant function in brain regions that handle the processing of visual-spatial data. These brain regions are necessary for writing, sketching, building models, and putting puzzle pieces together, as well as for perceiving a thing as a collection of parts. It is probable that the cytoskeleton, the structural framework that aids in determining cell shape, size, and mobility, is regulated in part by the LIMK1 protein within cells. Actin filaments are long, thin fibers that are a component of the cytoskeleton. The LIMK1 protein aids in the regulation of actin filament organization. The customized LIMK1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Emily

This LIMK1 KD cell line is highly selective. May 21 2020

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chat Rebecca

This mouse LIMK1 KD cell line can be applied well according to experimental needs. Jul 08 2021

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FAQ

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Published Data

Fig.1 Nuclear LIMK1 contributes to the aggressive phenotype of HCC cells in vitro.

The expression of LIMK1 in HCC cell lines was shown by Western blot analysis, and the relative protein abundance was calculated by normalizing to GAPDH.

Ref: Pan, Zhihua, et al. "LIMK1 nuclear translocation promotes hepatocellular carcinoma progression by increasing p-ERK nuclear shuttling and by activating c-Myc signalling upon EGF stimulation." Oncogene 40.14 (2021): 2581-2595.

Pubmed: 33686242

DOI: 10.1038/s41388-021-01736-2

Research Highlights

Actin dynamics is mostly regulated by LIM domain kinase 1 (LIMK1). Thus, it presents a possible therapeutic target for the avoidance of amyotrophic lateral sclerosis and fragile X syndrome.
Salah, Eidarus, et al. "Lessons from LIMK1 enzymology and their impact on inhibitor design." Biochemical Journal 476.21 (2019): 3197-3209.
Pubmed: 31652302 DOI: 10.1042/BCJ20190517

One common type of metastasis among individuals with advanced gastric cancer is peritoneal metastasis. The present investigation has revealed that LIM domain kinase 1 (LIMK1) is a promising therapeutic target for dabrafenib (DAB) and has been identified as a promoter of gastric cancer peritoneal metastasis.
Kang, Xi, et al. "LIMK1 promotes peritoneal metastasis of gastric cancer and is a therapeutic target." Oncogene 40.19 (2021): 3422-3433.
Pubmed: 33883692 DOI: 10.1038/s41388-021-01656-1