mProX™ Human LCK Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1190	Magic™ Human LCK in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

LCK

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

Ba/F3; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research; Immunology Research

Related Diseases

Immunodeficiency 22 and T-Cell Acute Lymphoblastic Leukemia. Among its related pathways are CD28 co-stimulation and "PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling"

Gene ID

3932

UniProt ID

P06239

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The 56 kDa protein Lck is present in lymphocytes, which are specific immune system cells. As a member of the Src kinase family (SFK), Lck plays a crucial role in naive T cells as well as effector T cells in activating T-cell receptor signaling. Compared to CD4 T cells, the activation or maintenance of memory CD8 T cells is less dependent on the Lck. Additionally, different memory T cell subsets have different roles for the lck. It appears that in mice, among the population of effector memory T cells, over 50% of lck exists in a constitutively active conformation, while less than 20% of lck exists in an active form. The customized LCK stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Linda

A very stable LCK KD cell line, especially as there has not been much variation change during my use. Dec 25 2022

chat Verified Customer

chat John

If you are looking for a good value LCK cell line for drug discovery, this is the one. Jul 11 2022

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 LCK expression promotes colony formation in vitro and tumor growth in vivo.

Western blotting was used to evaluate the total protein expression and flow cytometry was used to analyze FLT3 surface expression. It is an appropriate model to investigate the effect of LCK on FLT3-ITD mediated biological events because FLT3 surface and total expression seems to be the same for both LCK and empty vector expressing cells.

Ref: Marhäll, Alissa, Julhash U. Kazi, and Lars Rönnstrand. "The Src family kinase LCK cooperates with oncogenic FLT3/ITD in cellular transformation." Scientific reports 7.1 (2017): 13734.

Pubmed: 29062038

DOI: 10.1038/s41598-017-14033-4

Research Highlights

The various mechanisms that regulate Lck are thoroughly examined in this study, with an emphasis on how each process contributes to the maintenance of an acceptable level of Lck in each conformational state and, consequently, the efficient, suitable, and regulated amount of T cell activation that occurs after TCR stimulation.
Bozso, Sabin J., Jimmy JH Kang, and Jeevan Nagendran. "The role of competing mechanisms on Lck regulation." Immunologic Research 68.5 (2020): 289-295.
Pubmed: 32794043 DOI: 10.1007/s12026-020-09148-2

Tyrosine kinase (Lck), a protein exclusive to lymphocytes, has become known as one of the important molecules controlling T-cell activities in recent years. Research with Lck-deficient T-cell lines or knockout animals has demonstrated that Lck controls T-cell development, TCR signaling initiation, and T-cell homeostasis.
Bommhardt, Ursula, Burkhart Schraven, and Luca Simeoni. "Beyond TCR signaling: emerging functions of Lck in cancer and immunotherapy." International journal of molecular sciences 20.14 (2019): 3500.
Pubmed: 31315298 DOI: 10.3390/ijms20143500