mProX™ Human LATS2 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1189	Magic™ Human LATS2 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

LATS2

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research

Related Diseases

Malignant Peritoneal Mesothelioma and Malignant Pleural Mesothelioma. Among its related pathways are Epithelial to mesenchymal transition in colorectal cancer and Transcription co-factors SKI and SKIL protein partners

Gene ID

26524

UniProt ID

Q9NRM7

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The LATS2 gene in humans encodes the enzyme large tumor suppressor kinase 2 (LATS2). This gene participates in the Hippo signaling pathway, where it inactivates the effector proteins, YAP and WWTR1 (TAZ). It produces a serine/threonine protein kinase that is a member of the LATS tumor suppressor family. In both early and late metaphases as well as interphase, the protein localizes to centrosomes. It interacts with the centrosomal proteins ajuba and aurora-A and is necessary for spindle formation and gamma-tubulin accumulation at the beginning of mitosis. It may work in a positive feedback loop with p53 in response to cytoskeleton damage. It also interacts with a negative regulator of p53. It can also act as a corepressor of the expression of genes that respond to androgen. The customized LATS2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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The mouse LATS2 overexpression cell line proved to be an invaluable tool in my experiments. Mar 09 2022

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The LATS2 overexpression cell line I purchased was incredibly effective in my research. Jun 18 2021

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FAQ

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Published Data

Fig.1 LATS2 alterations in MPM cells in culture.

LATS2 mRNA and protein were expressed by MPM without mutation. Only those MPM exhibiting significant exon deletions showed complete reduction of mRNA expression. Only the MPM in culture with missense mutation (MPM_04) displayed LATS2 protein expression out of the seven LATS2 mutants.

Ref: Tranchant, Robin, et al. "Co-occurring mutations of tumor suppressor genes, LATS2 and NF2, in malignant pleural mesothelioma." Clinical Cancer Research 23.12 (2017): 3191-3202.

Pubmed: 28003305

DOI: 10.1158/1078-0432.CCR-16-1971

Research Highlights

According to available data, the MOB1 scaffold protein and the hippocampal kinases MST1/2 encourage the development of active MOB1/LATS complexes, which phosphorylate and inhibit YAP1.
Hoa, Lily, et al. "The characterisation of LATS2 kinase regulation in Hippo-YAP signalling." Cellular signalling 28.5 (2016): 488-497.
Pubmed: 26898830 DOI: 10.1016/j.cellsig.2016.02.012

According to this study, LATS2 O-GlcNAcylation plays a crucial part in the deregulation of the Hippo pathway in cancer cells, which is directly linked to the formation of tumors.
Kim, Eunah, et al. "O-GlcNAcylation on LATS2 disrupts the Hippo pathway by inhibiting its activity." Proceedings of the National Academy of Sciences 117.25 (2020): 14259-14269.
Pubmed: 32513743 DOI: 10.1073/pnas.1913469117