mProX™ Human LAG3 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- Immune Checkpoint Cell Lines
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Casey Williams (Verified Customer)
Patrick Liam (Creative Biolabs Scientific Support)
Taylor Miller (Verified Customer)
Patrick Liam (Creative Biolabs Scientific Support)
Published Data
Fig.1 Inflammatory activation is facilitated by the downregulation of Lymphocyte activation gene-3 (LAG-3) through the influence of IFN-γ.
Scramble control or LAG-3 siRNA was transfected into BV2 cells, followed by the addition of IFN-γ and subsequent cell harvesting. The efficiency of siRNA knockdown was confirmed by measuring LAG-3 mRNA levels using quantitative real-time PCR. Additionally, iNOS mRNA levels were assessed in the BV2 cells treated with siRNA, and the NO concentration in the culture supernatants of these cells was determined. Statistical analyses were carried out using a two-way ANOVA, followed by a Tukey-Kramer post-hoc test, revealing significant differences (∗∗p < 0.01, ∗∗∗∗p < 0.0001). SEM was used to represent the error bars in the data.
Ref: Morisaki, Yuta, et al. "LAG-3 expression in microglia regulated by IFN-γ/STAT1 pathway and metalloproteases." Frontiers in Cellular Neuroscience (2023).
Pubmed: NA
DOI: NA
Research Highlights
Hao, Yuanyuan. et al. "Tumor-derived exosomes induce initial activation by exosomal CD19 antigen but impair the function of CD19-specific CAR T-cells via TGF-β signaling." Frontiers of medicine, 2023.
Tumor-derived exosomes (TEXs) with immune suppressive properties primarily initiate T-cell dysfunction and hinder antitumor immunity. The potential influence of lymphoma TEXs on CAR T-cell therapy remains uncertain. In this study, it was found that B-cell lymphoma-derived exosomes initially activate CD19-CAR T-cells upon CD19 stimulation but may subsequently lead to CAR T-cell apoptosis and reduced tumor-killing ability due to increased expression of inhibitory receptors PD-1, TIM3, and LAG3 upon prolonged exposure. This phenomenon was also observed with plasma exosomes from lymphoma patients. Single-cell RNA sequencing revealed differentiated phenotypes and regulatory T-cell (Treg) conversion in CAR T-cells, which could be rescued by blocking TGF-β-Smad3 signaling with LY2109761. Combining CAR T-cell therapy with TGF-β inhibitors could offer a novel approach for treating refractory and relapsed B-cell lymphoma.
Hao, Yuanyuan. et al. "Tumor-derived exosomes induce initial activation by exosomal CD19 antigen but impair the function of CD19-specific CAR T-cells via TGF-β signaling." Frontiers of medicine, 2023.
Pubmed:
37870681
DOI:
10.1007/s11684-023-1010-1
Chen, Rukun. et al. "Short-term effects of PM2. 5 and its components exposure on endothelial function in Chinese elders." The Science of the total environment, 2023.
The presence of particulate matter (PM) in the atmosphere has been proven to have harmful effects on human health. PM is composed of tiny particles, such as dust, pollen, and soot, which can enter the respiratory system and potentially cause diseases. Exposure to PM has been linked to respiratory and cardiovascular issues, as well as premature death. Thus, understanding and addressing the sources and levels of PM is crucial for protecting human health.
Chen, Rukun. et al. "Short-term effects of PM2. 5 and its components exposure on endothelial function in Chinese elders." The Science of the total environment, 2023.
Pubmed:
37866598
DOI:
10.1016/j.scitotenv.2023.167909
