mProX™ Human KCNQ5 Stable Cell Line

Product Information

Target Protein

KCNQ5

Target Family

Kv7

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

Auditory and Otology Research; CNS Research

Related Diseases

Mental Retardation; Autosomal Dominant Non-Syndromic Intellectual Disability

Gene ID

56479

UniProt ID

Q9NR82

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The voltage-gated delayed rectifier potassium channels Kv7.1-7.5, which encode the α-subunits, are encoded by the five members of the highly conserved KCNQ gene family, including KCNQ5. It has been demonstrated that heteromeric channels containing Kv7.3 subunits along with KV7.5 or KV7.2 produce higher K+ currents than do each of the subunits alone. KCNQ1-4 is expressed in sensory outer hair cells6, KCNQ5 is found in the brain, skeletal muscle, and blood vessels, KCNQ1 is mostly expressed in cardiac muscle and the cochlea, and KCNQ2 and -3 are primarily expressed throughout the central nervous system (CNS) and peripheral nervous system (PNS). Apart from heteromerization, native KV7 currents can also be affected by assembly with KCNE subunits; mutations in either one of these might result in deafness or cardiac arrhythmia. The customized KCNQ5 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Linda

Flow cytometry is used to verify the surface expression of the KCNQ5 protein, and qPCR is used to verify the engineering results at the mRNA level. Jul 07 2021

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chat Patricia

The customized KCNQ5 stable cell line can be used in antibody discovery and development. Aug 07 2022

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FAQ

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Published Data

Fig.1 Aloperine activation of KCNQ5 requires KCNQ5-R212.

This suggested that aloperine attaches to the arginine at the base of the voltage sensor (R212 in KCNQ5), which is located deeper and across from W235 in KCNQ5 S5, much like mallotoxin does. KCNQ5 aloperine sensitivity was removed by the R212A mutation, even at doses up to 1 mM.

Ref: Manville, Rían W., et al. "KCNQ5 activation is a unifying molecular mechanism shared by genetically and culturally diverse botanical hypotensive folk medicines." Proceedings of the National Academy of Sciences 116.42 (2019): 21236-21245.

Pubmed: 31570602

DOI: 10.1073/pnas.1907511116

Research Highlights

Neuronal excitability is dampened by KCNQ2 (Kv7.2) and KCNQ3 (Kv7.3) K(+) channels, and dysfunction of these channels may result in epilepsy. KCNQ5 (Kv7.5), which likewise has widespread expression in the brain, is less well understood.
Fidzinski, Pawel, et al. "KCNQ5 K+ channels control hippocampal synaptic inhibition and fast network oscillations." Nature communications 6.1 (2015): 6254.
Pubmed: 25649132 DOI: 10.1038/ncomms7254

Tea causes vasodilation in part by activating KCNQ5; EGCG and ECG are potential targets for anti-hypertensive medication development in the future.
Redford, Kaitlyn E., et al. "KCNQ5 potassium channel activation underlies vasodilation by tea." Cellular physiology and biochemistry: international journal of experimental cellular physiology, biochemistry, and pharmacology 55.Suppl 3 (2021): 46.
Pubmed: 33667331 DOI: 10.33594/000000337