mProX™ Human KCNQ1 Stable Cell Line

Product Information

Target Protein

KCNQ1

Target Family

Kv7

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

Auditory and Otology Research; CNS Research

Related Diseases

Long Qt Syndrome; Jervell And Lange-Nielsen Syndrome

Gene ID

3784

UniProt ID

P51787

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

KV channel KCNQ1 has a variety of physiological functions. KCNQ1 supplies the cardiac action potential's delayed-rectifier IKs current when combined with the KCNE1 auxiliary protein. Heritable mutations in KCNQ1 result in loss of function or abnormal gain of function, which can induce a variety of arrhythmias, including long QT syndrome (LQTS). Conversely, KCNQ1 functions as a leak channel to help preserve ion homeostasis in epithelial cells when linked with accessory protein KCNE3. KCNQ1 conforms to the typical structural arrangement of the KV superfamily, where four VSDs, each with four transmembrane helix segments (S1-S4), flank the core homotetrameric pore domain. including other KV channels, including the Drosophila Shaker channel, each KCNQ1 VSD shows sequential activation. KCNQ1 is unique, though, in that it can conduct current when its VSDs occupy the intermediate conformation, even though Shaker and KCNQ1 can both conduct current when their VSDs adopt the activated conformation. The customized KCNQ1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Sharon

This KCNQ1 cell line is perfect for my experiments. Dec 03 2022

chat Verified Customer

chat Melissa

The Human KCNQ1 Stable Cell Line meets my requirement. Aug 30 2022

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Current traces of activation.

In Chinese Hamster Ovary (CHO) cells, wild-type KCNE1 and each mutant were transiently co-transfected with KCNQ1, and current was measured using a whole-cell patch clamp. The cells transfected with KCNQ1 and wild-type KCNE1 showed the well-known changed KCNQ1 current associated with IKs, in contrast to cells transfected with KCNQ1 alone.

Ref: Chen, Jerri, et al. "Functional interactions between KCNE1 C-terminus and the KCNQ1 channel." PLoS One 4.4 (2009): e5143.

Pubmed: 19340287

DOI: 10.1371/journal.pone.0005143

Research Highlights

Kv7.1, also known as KvLQT1, and KCNQ1 are members of the voltage-gated K(+) (Kv) channel superfamily. While KCNQ1 and other Kv channels share many common characteristics, KCNQ1's channel gating mechanism exhibits an amazing degree of flexibility that enables it to perform a variety of physiological functions in various tissues.
Liin, Sara I., Rene Barro-Soria, and H. Peter Larsson. "The KCNQ1 channel-remarkable flexibility in gating allows for functional versatility." The Journal of physiology 593.12 (2015): 2605-2615.
Pubmed: 25653179 DOI: 10.1113/jphysiol.2014.287607

Auxiliary subunit KCNE proteins significantly control the potassium channel KCNQ1's gating. While it is well known that KCNE proteins interact with KCNQ1 through the pore domain, several recent results indicate that KCNE may also modify the VSD movement.
Nakajo, Koichi, and Yoshihiro Kubo. "KCNQ1 channel modulation by KCNE proteins via the voltage-sensing domain." The Journal of physiology 593.12 (2015): 2617-2625.
Pubmed: 25603957 DOI: 10.1113/jphysiol.2014.287672