mProX™ Human KCNK9 Stable Cell Line

Product Information

Target Protein

KCNK9

Target Family

TASK

Target Protein Species

Human

Host Cell Type

PASMC; COS7; CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

Cardiovascular Research; CNS Research

Related Diseases

Birk-Barel Syndrome; Kcnk9 Imprinting Syndrome

Gene ID

51305

UniProt ID

Q9NPC2

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The KCNK9 gene codes for the production of the potassium channel-functioning protein TASK3. There are TASK3 channels all over the body. They are particularly prevalent in the brain's nerve cells, notably in the area responsible for motor coordination. While TASK3 channels are always open, their activity can be influenced by the environment around the cell, in contrast to particular potassium channels that open and close in response to specific stimuli. The channels are frequently referred to as background or leak channels since they are always open. TASK3 channels preserve a cell's capacity to produce electrical impulses and control cell activity. The brain's neuronal mobility appears to be influenced by these channels as well. The customized KCNK9 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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I customized a KCNK9 knockdown cell line and it meets my requirement. May 05 2020

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The quality of work, efficiency, and speed was perfect. Jun 13 2023

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FAQ

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Published Data

Fig.1 Mutant KCNK3 channels in COS7 cells.

Normal voltage clamp recordings of V221L (bottom) and wildtype (WT) KCNK3. Sample current traces are displayed for pH values of 6.4 (blue), 7.4 (black), and 10.4 (red). For each voltage clamp recording, a voltage ramp (top) was applied, ranging from -120 mV to +60 mV over 0.5 s, every 3 s. The holding potential was set at -80 mV.

Ref: Bohnen, Michael S., et al. "The Impact of Heterozygous KCNK 3 Mutations Associated With Pulmonary Arterial Hypertension on Channel Function and Pharmacological Recovery." Journal of the American Heart Association 6.9 (2017): e006465.

Pubmed: 28889099

DOI: 10.1161/JAHA.117.006465

Research Highlights

By showing the intricate etiology of KIS, which includes gain and loss of channel function as well as a persistent loss of channel control, this study adds to our understanding of the mechanisms underlying KIS.
Cousin, Margot A., et al. "Gain and loss of TASK3 channel function and its regulation by novel variation cause KCNK9 imprinting syndrome." Genome medicine 14.1 (2022): 62.
Pubmed: 35698242 DOI: 10.1186/s13073-022-01064-4

Congenital hypotonia, varied cleft palate, normal MRIs and EEGs, delayed development, and feeding issues are all present in patients with KCNK9 imprinting syndrome.
Graham Jr, John M., et al. "KCNK9 imprinting syndrome-further delineation of a possible treatable disorder." American Journal of Medical Genetics Part A 170.10 (2016): 2632-2637.
Pubmed: 27151206 DOI: 10.1002/ajmg.a.37740