mProX™ Human KCNK2 Stable Cell Line

Product Information

Target Protein

KCNK2

Target Family

TREK

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

Cardiovascular Research; CNS Research

Related Diseases

Dentin Sensitivity; Autosomal Recessive Limb-Girdle Muscular Dystrophy Type 2X

Gene ID

3776

UniProt ID

O95069

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The KCNK2 gene in humans encodes TREK-1, a protein that is a member of the potassium channel subfamily K. K2P2.1, a lipid-gated ion channel that is a member of the two-pore-domain background potassium channel protein family, is encoded by this gene. Two homodimers combine to form this type of potassium channel, which controls the resting membrane potential by releasing potassium out of the cell. Anionic lipids, certain anesthetics, stretching of the membrane, intracellular acidosis, and heat all open the channel. For this gene, three transcript variants encoding distinct isoforms have been discovered. Mammalian neurons have mechano-gated potassium channels, among which TREK-1 is a subfamily. Both chemical and physical cues can be used to open them, and they can be gated in both ways. While TREK-1 channels are present in many different tissues, they are most prevalent in the heart and brain and are located in different kinds of neurons. The mechanosensitivity of TREK-1 channels is influenced by their C-terminal. The customized KCNK2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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I customized a KCNK2 cell line and the products definitely stand out. Aug 18 2023

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We are quite happy with the knockdown cells that you made. Mar 19 2022

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FAQ

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Published Data

Fig.1 Expression and localization of KCNK2, KCNK10, KCNK4, KCNK3, and KCNK9 in bovine oocytes and blastocysts.

Intensity of KCNK2 immunofluorescence, KCNK10, KCNK4, KCNK3, and KCNK9. The mean G.S.E.M. of ten independent experiments is represented by each data point. In the blastocyst stage, there was a considerable decrease in the proteins KCNK10 and KCNK4. Mature oocytes stopped at metaphase II and blastocyst, respectively, are denoted as MII and BL.

Ref: Hur, Chang-Gi, et al. "Expression and localization of two-pore domain K+ channels in bovine germ cells." Reproduction 137.2 (2009): 237-244.

Pubmed: 18987255

DOI: 10.1530/REP-08-0035

Research Highlights

In 590 MDD patients and 441 controls, six single nucleotide polymorphisms (SNPs) of the KCNK2 gene were examined for their relationship to major depressive disorder (MDD) and treatment effectiveness.
Congiu, Chiara, et al. "The role of the potassium channel gene KCNK2 in major depressive disorder." Psychiatry Research 225.3 (2015): 489-492.
Pubmed: 25535009 DOI: 10.1016/j.psychres.2014.11.061

Members of the potassium two pore domain channel family K, or KCNKs, are able to modify the membrane potential and membrane excitability in addition to maintaining the resting potential and controlling the amplitude and length of the action potential plateau.
Lin, Xu, et al. "The correlation and role analysis of KCNK2/4/5/15 in Human Papillary Thyroid Carcinoma microenvironment." Journal of Cancer 11.17 (2020): 5162.
Pubmed: 32742463 DOI: 10.7150/jca.45604