mProX™ Human KCNJ2 Stable Cell Line

Product Information

Target Protein

KCNJ2

Target Family

GIRK

Target Protein Species

Human

Host Cell Type

SCLC H69; H446; CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

Cardiovascular Research; CNS Research

Related Diseases

Andersen Cardiodysrhythmic Periodic Paralysis; Short Qt Syndrome

Gene ID

3759

UniProt ID

P63252

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The KCNJ2 gene is a member of a broad gene family that codes for the production of potassium channels. Both cardiac muscle and muscles used for movement have active KCNJ2 protein-made channels. These channels are crucial to the pattern of muscular tensing and relaxation in skeletal muscle, which permits movement. The channels in the heart are responsible for replenishing the cardiac muscle following each beat in order to preserve a steady rhythm. Although their precise function in bone growth is unknown, channels made with the KCNJ2 protein may also be involved in this process. Scientists have discovered that for channels produced with the KCNJ2 protein to operate properly, a molecule known as PIP2 needs to connect to the channel. The customized KCNJ2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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I purchased the KCNJ2 cell line. Hope it will work well. Apr 01 2022

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I was so pleased with the KCNJ2 cell line I received from this business. Apr 09 2022

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FAQ

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Published Data

Fig.1 KCNJ2/Kir2.1 expressionin SCLC cells.

In comparison to H69 and H446 cells, respectively, KCNJ2/Kir2.1 expression was considerably higher in H69AR and H446AR cells both at the mRNA and protein levels. Kir2.1 expression was elevated in H69AR and H44AR cells as compared with H69 and H446 cells, according to Western blotting and immunofluorescence studies.

Ref: Liu, Huanxin, et al. "Upregulation of the inwardly rectifying potassium channel Kir2. 1 (KCNJ2) modulates multidrug resistance of small-cell lung cancer under the regulation of miR-7 and the Ras/MAPK pathway." Molecular cancer 14.1 (2015): 1-19.

Pubmed: 25880778

DOI: 10.1186/s12943-015-0298-0

Research Highlights

Numerous cardiovascular diseases have previously been linked to inwardly rectifying potassium channels (Kir). Specifically, patients with Andersen-Tawil syndrome have been linked to loss-of-function mutations in the Kir2.1 channel, whereas short QT3 syndrome is caused by gain-of-function mutations in the same channel.
Binda, Anna, et al. "A novel KCNJ2 mutation identified in an autistic proband affects the single channel properties of Kir2. 1." Frontiers in cellular neuroscience 12 (2018): 76.
Pubmed: 29615871 DOI: 10.3389/fncel.2018.00076

The autosomal dominant hereditary or sporadic Andersen-Tawil syndrome (ATS) is typified by dysmorphic features, ventricular arrhythmias (VAs), and recurrent paralyses. It is unknown what the best pharmaceutical regimen is for treating VAs in patients with ATS.
Miyamoto, Koji, et al. "Efficacy and safety of flecainide for ventricular arrhythmias in patients with Andersen-Tawil syndrome with KCNJ2 mutations." Heart Rhythm 12.3 (2015): 596-603.
Pubmed: 25496985 DOI: 10.1016/j.hrthm.2014.12.009