mProX™ Human KCNH3 Stable Cell Line

Product Information

Target Protein

KCNH3

Target Family

Kv7

Target Protein Species

Human

Host Cell Type

SKOV3; COC1; CHO-K1; HEK293

Accession Number

NM_012284

Target Classification

Ion Channel Cell Lines

Target Research Area

Auditory and Otology Research; CNS Research

Related Diseases

Deafness; Autosomal Dominant 2A

Gene ID

23416

UniProt ID

Q9ULD8

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The voltage-gated potassium (K+) channel KCNH3 is a member of the ether-a-go-go (KCNH) family. This family of channels produces an outward current that has both transient and steady-state components that are dependent on voltage. The steady component exhibits a bell-shaped current-voltage relationship, which is also seen in the transcriptional regulator ERG, a member of the KCNH family, while the transient component possesses fast-inactivating kinetics. One of the factors influencing neuronal excitability is the genetically diverse K+ channels, which are mostly expressed in the central nervous system. Although K+ channel blockers have been shown to have therapeutic promise for improving cognitive function, it is still unknown how much each K+ channel gene contributes to cognitive processes. Thanks to a particular expression in the cell bodies of neurons with characteristic pyramidal forms, KCNH3 is broadly expressed in cerebral cortexes from layer II to layer VI. Subsequent research revealed that it is related to the human central nervous system's constrained neurons' cellular excitability. The customized KCNH3 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

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FAQ

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Published Data

Fig.1 Inhibition of KCNH3 affects ovarian cancer cell proliferation and migration in vitro.

Comparing the SKOV3 and COC1 cell lines in the KCNH3-silencing groups to the control groups (NC), clear inhibitory effects on cell proliferation were seen. In both SKOV3 and COC1 cancer cells, KCNH3 silencing groups showed a notable G2/M phase block. These findings imply that KCNH3 may promote OC cell proliferation.

Ref: Li, Zhongjun, et al. "KCNH3 predicts poor prognosis and promotes progression in ovarian cancer." OncoTargets and therapy (2020): 10323-10333.

Pubmed: 33116612

DOI: 10.2147/OTT.S268055

Research Highlights

A voltage-gated K+ channel belonging to the ether-à-go-go (KCNH) family is KCNH3 (BEC1). Determining the pharmacological characteristics of ASP2905, a KCNH3 inhibitor, both in vitro and in vivo was the goal of this investigation.
Takahashi, Shinji, et al. "Neurochemical and neuropharmacological characterization of ASP2905, a novel potent selective inhibitor of the potassium channel KCNH3." European Journal of Pharmacology 810 (2017): 26-35.
Pubmed: 28552344 DOI: 10.1016/j.ejphar.2017.05.045

Mice with overexpression of KCNH3, which is localized in the forebrain, have cognitive impairments. On the other hand, Kcnh3 knockout mice do better on cognitive tasks like attentiveness. These findings imply that KCNH3 is involved in cognitive processes.
Takahashi, Shinji, et al. "The KCNH3 inhibitor ASP2905 shows potential in the treatment of attention deficit/hyperactivity disorder." PLoS One 13.11 (2018): e0207750.
Pubmed: 30462746 DOI: 10.1371/journal.pone.0207750