mProX™ Human KCNC1 Stable Cell Line

Product Information

Target Protein

KCNC1

Target Family

Kv3

Target Protein Species

Human

Host Cell Type

HT; NT2; CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

Cardiovascular Research; CNS Research

Related Diseases

Epilepsy; Progressive Myoclonic; Spinocerebellar Ataxia

Gene ID

3746

UniProt ID

P48547

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Four genes make up the Kv3 subfamily: Kv3.1 (also called KCNC1), Kv3.2, Kv3.3, and Kv3.4. By alternatively splicing the 3' ends of each Kv3 gene, numerous products that differ only in their short C-terminal sequences are expressed. Alternative splicing of the 3' ends of additional K+ channel genes may play a similar role since the different C termini include or control targeting signals. Kv3-type channels are present in inhibitory GABAergic interneurons that also produce parvalbumin, a calcium-binding protein that serves as a marker for fast-spiking neurons in the neocortex, thalamus, hippocampus, and striatum. Kv3.1 channels, however, have also been found in astrocytes, T-lymphocytes, oligodendrocyte precursor cells, and NPCs in addition to neurons. The customized KCNC1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Cynthia

Very easy to use for drug screening. Aug 29 2020

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Excellent service! Very reliable. Fast turnaround time. Nov 02 2021

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FAQ

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Published Data

Fig.1 Aberrant KCNC1 affects the invasion and metastasis of human testis Hs1.Tes (HT) and Ntera-2 testicular tumor (NT2) cells.

KCNC1 mRNA and protein expression levels in the corresponding cells after KCNC1 overexpression and KCNC1-specific siRNA knockdown. Western blot analysis was used to confirm the expression of markers related to the epithelial-mesenchymal transition when KCNC1 was knocked down in human testis HT and overexpressed in Ntera-2 testicular tumor (NT2) cells.

Ref: Chen, Saipeng, et al. "Methylation gene KCNC1 is associated with overall survival in patients with seminoma." Oncology Reports 45.5 (2021): 1-10.

Pubmed: 34105734

DOI: 10.3892/or.2021.8024

Research Highlights

A unique class of seizure disorders known as progressive myoclonus epilepsy (PME) is typified by a progressive neurological decline accompanied by myoclonus, ataxia, and recurrent seizures.
Nascimento, Fábio A., and Danielle M. Andrade. "Myoclonus epilepsy and ataxia due to potassium channel mutation (MEAK) is caused by heterozygous KCNC1 mutations." Epileptic Disorders 18.s2 (2016): S135-S138.
Pubmed: 27629860 DOI: 10.1684/epd.2016.0859

A class of uncommon, hereditary illnesses known as progressive myoclonus epilepsies (PMEs) are characterized by ataxia, tonic-clonic seizures, and action myoclonus.
Muona, Mikko, et al. "A recurrent de novo mutation in KCNC1 causes progressive myoclonus epilepsy." Nature genetics 47.1 (2015): 39-46.
Pubmed: 25401298 DOI: 10.1038/ng.3144