mProX™ Human KCNA5 Stable Cell Line

Product Information

Target Protein

KCNA5

Target Family

Kv1

Target Protein Species

Human

Host Cell Type

COS-7; rPASMC; rMASMC; CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

Cardiovascular Research; CNS Research; Digestive and Renal Research

Related Diseases

Atrial Fibrillation

Gene ID

3741

UniProt ID

P22460

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The fact that Kv1.5 antisense oligodeoxynucleotides exclusively reduce IKur density in human atrial myocytes and the fact that Kv1.5 gene is significantly expressed in the human atrium support the notion that Kv1.5 (KCNA5) encodes the pore-forming subunit of human atrial IKur. IKur is initially discovered to be encoded by Kv3.1 in the canine atrium, however a subsequent investigation has confirmed that Kv1.5 does indeed encode the IKur in canine atrial myocytes. Due to the results of the subsequent investigation, it is now conceivable to test IKur/Kv1.5 blockers for the treatment of AF in dogs. Additionally, mouse and rat hearts contain the Kv1.5 mRNA and protein, which are in charge of IKur. Additionally, Kv1.5 transcripts have been found in extracardiac organs such as the pituitary, brain, pancreas, and others. The customized KCNA5 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Laura

Flow cytometry is used to verify the surface expression of the KCNA5 protein, and qPCR is used to verify the engineering results at the mRNA level. Aug 23 2023

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chat Michelle

The customized KCNA5 stable cell line can be used in antibody discovery and development. Dec 17 2020

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FAQ

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Published Data

Fig.1 Structure of KCNA5 gene and characteristics of Kv1.5 channel currents.

Representative single channel Kv1.5 currents from a human embryonic kidney (HEK)-293 cell, a rat PASMC, a human coronary artery smooth muscle cell (CASMC) transfected with KCNA5, and a COS-7 cell at a test potential (TP) of 100 mV.

Ref: Remillard, Carmelle V., et al. "Function of Kv1. 5 channels and genetic variations of KCNA5 in patients with idiopathic pulmonary arterial hypertension." American Journal of Physiology-Cell Physiology 292.5 (2007): C1837-C1853.

Pubmed: 17267549

DOI: 10.1152/ajpcell.00405.2006

Research Highlights

Cancer cells have PcG-dependent chromatin repressive changes that become more pronounced in hypoxic environments, marking the KCNA5 promoter. KCNA5 expression is restored by genetic and pharmacological suppression of BMI-1 and EZH2, respectively, making cells more susceptible to stress-induced death.
Ryland, Katherine E., et al. "Polycomb-dependent repression of the potassium channel-encoding gene KCNA5 promotes cancer cell survival under conditions of stress." Oncogene 34.35 (2015): 4591-4600.
Pubmed: 25435365 DOI: 10.1038/onc.2014.384

One typical feature of human or experimental pulmonary arterial hypertension (PAH) is reduced expression and/or activity of Kv1.5 channels. Similarly, genetic variations in KCNA5 have been discovered in PAH patients; however, little is known about these variants' functional implications and possible influence on the illness.
Vera-Zambrano, Alba, et al. "Novel loss-of-function KCNA5 variants in pulmonary arterial hypertension." American Journal of Respiratory Cell and Molecular Biology 69.2 (2023): 147-158.
Pubmed: 36917789 DOI: 10.1165/rcmb.2022-0245OC