mProX™ Human KCNA10 Stable Cell Line

Product Information

Target Protein

KCNA10

Target Family

Kv1

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

Cardiovascular Research; CNS Research

Related Diseases

Developmental And Epileptic Encephalopathy; Episodic Ataxia

Gene ID

3744

UniProt ID

Q16322

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Membrane proteins called potassium (K) channels play a key role in many physiological processes by controlling membrane potential. The most distinctive characteristic of the voltage-gated K (Kv) channel gene KCNA10, which belongs to the Shaker superfamily, is the presence of a putative cyclic nucleotide-binding (CNB) domain at the carboxy terminus. Voltage-gated K currents are mediated by the KCNA10 α subunit, which shows little steady-state inactivation. More positive depolarizations than 66 mV cause channels to open. The channel has an interesting inhibitor profile since it is sensitive to drugs that inhibit cyclic nucleotide gated cation channels, such verapamil and pimozide, in addition to being blocked by traditional K channel blockers. According to tail current data, KCNA10 has a selectivity ratio of at least 15:1 for potassium to sodium. The customized KCNA10 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Donna

The KCNA10 cell line worked very well. Sep 02 2023

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chat Charles

I customized a KCNA10 overexpression cell line and it was verified that the product meets my requirement. Nov 26 2020

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FAQ

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Published Data

Fig.1 KCNA10 is a voltage-gated K channel.

The 2-electrode voltage clamp was used to record the whole cell KCNA10 currents from a Xenopus laevis oocyte. The results indicate that KCNA10 functions as a voltage-gated channel, with a threshold voltage of around −10 mV. Observe that there is little steady-state inactivation seen in the ensemble KCNA10 currents.

Ref: Lang, Rainer, et al. "KCNA10: a novel ion channel functionally related to both voltage-gated potassium and CNG cation channels." American Journal of Physiology-Renal Physiology 278.6 (2000): F1013-F1021.

Pubmed: 10836990

DOI: 10.1152/ajprenal.2000.278.6.F1013

Research Highlights

Global rise in the prevalence of endemic chronic kidney disease of unknown etiology (CKDu) possess major health issues. The prevalence of CKDu is also rising in the Indian population. Besides environmental factors, genetic factors play an important role in the predisposition to CKDu.
Kumari, Ravita, et al. "Association of Single Nucleotide Polymorphisms in KCNA10 and SLC13A3 Genes with the Susceptibility to Chronic Kidney Disease of Unknown Etiology in Central Indian Patients." Biochemical Genetics (2023): 1-19.
Pubmed: 36696070 DOI: 10.1007/s10528-023-10335-7

Congenital long QT syndrome (LQTS) is one type of inherited fatal cardiac arrhythmia that may lead to sudden cardiac death (SCD). Mutations in more than 16 genes have been reported to be associated with LQTS, whereas the genetic causes of about 20% of cases remain unknown.
Huang, Shuainan, et al. "Whole-exome sequencing and electrophysiological study reveal a novel loss-of-function mutation of KCNA10 in epinephrine provoked long QT syndrome with familial history of sudden cardiac death." Legal Medicine 62 (2023): 102245.
Pubmed: 36965351 DOI: 10.1016/j.legalmed.2023.102245