mProX™ Human HTR5A Stable Cell Line

Product Information

Target Protein

HTR5A

Target Family

5-Hydroxytryptamine Family

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

GPCR Cell Lines

Target Research Area

Cardiovascular Research; CNS Research

Related Diseases

Attention Deficit-Hyperactivity Disorder; Sacral Defect With Anterior Meningocele

Gene ID

3361

UniProt ID

P47898

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The 5-hydroxytryptamine has both vasoconstrictive and vasodilatory actions, and it has been linked to a number of psychiatric disorders. The 5-hydroxytryptamine receptor HTR5A interacts to G proteins to have an adverse effect on cAMP levels through Gi and Go. It has been hypothesized that this receptor works in part by controlling intracellular Ca2+ mobilization. It has been demonstrated that HTR5A works properly in a native expression system. As a presynaptic serotonin autoreceptor, it also seems to function. It has been discovered that a selective HTR5A receptor antagonist lowers 5-carboxyamidotryptamine-induced inhibition of raphe neuronal cell firing in vitro and increases 5-HT levels in prefrontal cortex in vivo, pointing to a role for the 5-HT5A receptor in the modulation of raphe 5-HT neuronal cell firing. The customized HTR5A stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Una (Verified Customer)

How do I select the right host cell? Apr 25 2023

chat Sherry Smith (Creative Biolabs Scientific Support)

You'll need to take into account your research objectives. A more physiologically appropriate cell type may be the ideal choice to achieve more pertinent results if you are examining cellular metabolism or the effects of particular nutrients on cellular activities. Apr 25 2023

chat Truda (Verified Customer)

Can I request a specific host cell for the HTR5A stable cell line? Jun 07 2023

chat Sherry Smith (Creative Biolabs Scientific Support)

Yes, you can select suitable host cells for customized cell line. Jun 07 2023

Published Data

Fig.1 Dose-response split TEV assay for the serotonin receptor HTR5A using the agonist serotonin.

Increasing serotonin concentrations triggered the HTR5A serotonin receptor. Serotonin concentration of 160 nM was used to get the EC 50 value (gray lines). Transient fusions of HTR5A-V2R-NTEV-tevS-GV and ARRB2-CTEV were observed.

Ref: Wehr, Michael C., Sabrina Galinski, and Moritz J. Rossner. "Monitoring G protein-coupled receptor activation using the protein fragment complementation technique split TEV." G Protein-Coupled Receptor Screening Assays: Methods and Protocols (2015): 107-118.

Pubmed: 25563180

DOI: 10.1007/978-1-4939-2336-6_8

Research Highlights

Two serotonin (S) receptors, HTR2c and HTR5a, were shown to be expressed and functionally up-regulated by cortisol, according to the analysis. When pre-adipocytes were cultured alone or with macrophages present, HTR2c and HTR5a were also directly implicated in mediating cortisol accelerated adipogenesis.
Priyadarshini, Sushri, et al. "Cortisol regulates immune and metabolic processes in murine adipocytes and macrophages through HTR2c and HTR5a serotonin receptors." European Journal of Cell Biology 97.7 (2018): 483-492.
Pubmed: 30097291 DOI: 10.1016/j.ejcb.2018.07.004

This study discovered that by boosting innate immune signals, valerenic acid can prevent GBM cells from proliferating, migrating, and invading. Valerenic Acid-induced cell death might be prevented by N-acetylcysteine's (NAC) inhibition of ROS or Compound C's attenuation of AMPK.
Lu, Qingli, Yuan Ding, and Yang Li. "5-HT receptor agonist Valerenic Acid enhances the innate immunity signal and suppresses glioblastoma cell growth and invasion." International Journal of Biological Sciences 16.12 (2020): 2104.
Pubmed: 32549758 DOI: 10.7150/ijbs.44906