mProX™ Human HTR4 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX928	Magic™ Rat HTR4 in Vitro cAMP Assay	Rat	CHO-K1	cAMP Assay

Product Information

Target Protein

HTR4

Target Family

5-Hydroxytryptamine Family

Target Protein Species

Human

Host Cell Type

COS7; CHO-K1; HEK293

Target Classification

GPCR Cell Lines

Target Research Area

CNS Research; Digestive and Renal Research

Related Diseases

Irritable Bowel Syndrome; Constipation

Gene ID

3360

UniProt ID

Q13639

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

HTR4 receptors regulate the release of numerous neurotransmitters, including glutamate, GABA, dopamine, epinephrine/norepinephrine, and acetylcholine, as well as many hormones, including oxytocin, prolactin, vasopressin, and cortisol. They also govern both excitatory and inhibitory neurotransmission. By regulating the release of different neurotransmitters, HTR4 affects both peripheral and central nervous system processes such as the sleep-wake cycle, thermoregulation, food intake, nociception, locomotion, sexual behavior, gastrointestinal motility, blood coagulation, and cardiovascular homeostasis. G proteins that stimulate adenylate cyclase and aid in the production of cAMP mediate the activity of HTR4. HTR4 is thought to intensify inflammatory pain and has been linked to inflammatory hyperalgesia. In mouse models of depression, studies have demonstrated that an HTR4 agonist partially exhibits antidepressant-like effects. The customized HTR4 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Channing (Verified Customer)

Can the HTR4 cell line be used in PPI studies? Feb 19 2023

chat Sherry Smith (Creative Biolabs Scientific Support)

Yes, cell lines expressing specific proteins can be used in PPI studies. Feb 19 2023

chat Sigrid (Verified Customer)

Does the HTR4 stable cell line overexpress the full-length HTR4? Sep 02 2023

chat Sherry Smith (Creative Biolabs Scientific Support)

Yes, the HTR4 stable cell line can be customized to overexpress the full-length HTR4. Sep 02 2023

Published Data

Fig.1 p11 increases 5-HTR4 expression at the plasma membrane.

In comparison to the entire hippocampus lysate from p11 WT or KO animals, 5-HTR4 protein expression was found in the plasma membrane enriched fraction (left, Western blot; right, quantification).

Ref: Warner-Schmidt, Jennifer L., et al. "Role of p11 in cellular and behavioral effects of 5-HT4 receptor stimulation." Journal of Neuroscience 29.6 (2009): 1937-1946.

Pubmed: 19211900

DOI: 10.1523/JNEUROSCI.5343-08.2009

Research Highlights

According to this study, 5-HTR3 and 5-HTR4 were found in the cardiac mitochondria and regulated both cellular and mitochondrial activity. In newborn cardiomyocytes, 5-HTR3 and 5-HTR4 knockdown significantly increased cell damage in response to hypoxia and also caused heartbeat alternation.
Wang, Qingyi, et al. "5-HTR3 and 5-HTR4 located on the mitochondrial membrane and functionally regulated mitochondrial functions." Scientific reports 6.1 (2016): 37336.
Pubmed: 27874067 DOI: 10.1038/srep37336

By using immunofluorescence labeling, the current work examined the location of these HTRs in the mouse gut. HTR2A, HTR2B, and HTR4 were found in the epithelium's solitary cells, while HTR2C was found in the lamina propria.
Okumura, Motoshi, et al. "Expression of serotonin receptor HTR4 in glucagon-like peptide-1-positive enteroendocrine cells of the murine intestine." Pflügers Archiv-European Journal of Physiology 472 (2020): 1521-1532.
Pubmed: 32870377 DOI: 10.1007/s00424-020-02453-7