mProX™ Human HTR3A Stable Cell Line

Product Information

Target Protein

HTR3A

Target Family

5-Hydroxytryptamine Family

Target Protein Species

Human

Host Cell Type

DLD-1; HCT116; SW1116; SW620; CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research; Reproductive Research

Related Diseases

Serotonin Syndrome; Motion Sickness

Gene ID

3359

UniProt ID

P46098

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Serotonin, also known as 5-hydroxytryptamine (5-HT), was first discovered in the blood as a vasoconstrictor. The ligand-gated cation channel subunit may be encoded by the 5-HT3 subtype, which contains three subunits: 5-HT3A (also known as HTR3A), 5-HT3B (also known as HTR3B), and HTR3C. As a homo-pentamer, the HTR3A subunit creates a functional channel. In the six CRC cell lines, HTR3A expression varied. Additionally, HTR3A knockdown prevented CRC cells from proliferating and forming colonies, which induced cell cycle arrest and encouraged cell death. Additionally, following HTR3A knockdown, the expression of apoptosis-related proteins like BAD and BAX was elevated whereas Bcl2 expression was lowered. The customized HTR3A stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Philip (Verified Customer)

What host cells are typically used for the HTR3A stable cell line? Aug 18 2022

chat Sherry Smith (Creative Biolabs Scientific Support)

Mammalian cells such as HEK293 or CHO-K1. Aug 18 2022

chat Burgess (Verified Customer)

Is it possible to infer information about the important cell type from the STR profile? Nov 05 2022

chat Sherry Smith (Creative Biolabs Scientific Support)

Yes, but to distinguish between different cell populations or cell types, cell clusters must first be manually annotated using flag genes unique to the animal or tissue under study. Nov 05 2022

Published Data

Fig.1 HTR3A is efficiently knocked down in CRC cell lines.

Cell lines CRC HCT116 and SW1116 were infected for 96 hours with lentiviruses producing shCon, shHTR3A(S1), or shHTR3A(S2). A fluorescent microscope was used to take the representative bright-field (top panel) and fluorescent-field (lower panel) microscopy pictures (magnification, 100).

Ref: Tang, Jian, et al. "Downregulation of 5‑hydroxytryptamine receptor 3A expression exerts an anticancer activity against cell growth in colorectal carcinoma cells in vitro." Oncology letters 16.5 (2018): 6100-6108.

Pubmed: 30405756

DOI: 10.3892/ol.2018.9351

Research Highlights

These findings imply that the 5-HT3 receptor may be a promising target for the treatment of stress-related diseases since it enhances SSRI efficacy and inhibits the onset of stress-induced harmful consequences.
Martin, Vincent, et al. "Response of Htr3a knockout mice to antidepressant treatment and chronic stress." British journal of pharmacology 174.15 (2017): 2471-2483.
Pubmed: 28493335 DOI: 10.1111/bph.13857

These findings imply that the 5-HT3 receptor may be a promising target for the treatment of stress-related diseases since it enhances SSRI efficacy and inhibits the onset of stress-induced harmful consequences.
Tone, Mitsuyo, et al. "HTR3A is correlated with unfavorable histology and promotes proliferation through ERK phosphorylation in lung adenocarcinoma." Cancer science 111.10 (2020): 3953-3961.
Pubmed: 32736413 DOI: 10.1111/cas.14592