mProX™ Human HTR1E Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX896	Magic™ Dog HTR1E in Vitro Radioligand Binding Assay	Dog	CHO-K1	Radioligand Binding Assay
S01YF-1122-KX900	Magic™ Rabbit HTR1E in Vitro Radioligand Binding Assay	Rabbit	CHO-K1	Radioligand Binding Assay

Product Information

Target Protein

HTR1E

Target Family

5-Hydroxytryptamine Family

Target Protein Species

Human

Host Cell Type

LN-18; CHO-K1; HEK293

Target Classification

GPCR Cell Lines

Target Research Area

CNS Research

Related Diseases

Attention Deficit-Hyperactivity Disorder; Autism Spectrum Disorder

Gene ID

3354

UniProt ID

P28566

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Serotonin's G-protein coupled receptor, HTR1E (5-HT1E), performs this function. Additionally, it can serve as a receptor for several alkaloids and psychotropic drugs. When 5-HT1E interacts with its ligand 5-hydroxytryptamine, a conformational shift occurs that initiates signaling via guanine nucleotide-binding proteins and controls the activity of downstream effectors such adenylate cyclase. Adenylate cyclase activity is inhibited by 5-HT1E activation. It is possible to use 5-HT1E as a drug target for the treatment of neuropsychiatric disorders characterized by memory deficits or as a target for the treatment of temporal lobe epilepsy due to the stimulation of 5-HT1E and subsequent inhibition of adenylate cyclase activity in the dentate gyrus (DG). This finding suggests that 5-HT1E may mediate regulation of hippocampal activity by 5-HT. The customized HTR1E stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Colbert (Verified Customer)

Why are my cells replicating very slowly? Sep 23 2022

chat Sherry Smith (Creative Biolabs Scientific Support)

Keep in mind that engineered cells are known to reproduce more slowly than their unmodified mother cells. Additionally, confirm that you are using the appropriate media reagents for your cell line. If so, the following are some additional frequent causes:
Adding antibiotics to a thawed culture prematurely.
Spinning down the thawed cells to remove DMSO.
Over-splitting.
Clumping. Sep 23 2022

chat Tobey (Verified Customer)

What do I do if my signal-to-background is too low? Dec 04 2022

chat Sherry Smith (Creative Biolabs Scientific Support)

Examine the condition of your cells. Verify that all culture or plating methods were followed and that the cells arrived frozen.
The high protein concentration of crude lysates may be hindering the experiment if you're utilizing them. Dec 04 2022

Published Data

Fig.1 Co-immunoprecipitation experiments.

Using 5-HTR1E or CPE antibodies, LN-18 cell extracts were immunoprecipitated (IP), followed by immunoblotting with anti-CPE or anti-5-HTR1E antibodies; the arrowhead denotes co-precipitated CPE and 5-HTR1E protein bands. Positive controls included whole cell lysate (WCE), whereas IP controls included regular rabbit or mouse serum (IP Ctrl).

Ref: Sharma, Vinay Kumar, et al. "Novel interaction between neurotrophic factor-α1/carboxypeptidase E and serotonin receptor, 5-HTR1E, protects human neurons against oxidative/neuroexcitotoxic stress via β-arrestin/ERK signaling." Cellular and Molecular Life Sciences 79.1 (2022): 24.

Pubmed: 36808009

DOI: 10.1007/s00018-023-04711-0

Research Highlights

It has been extensively researched how stress chemicals, particularly glucocorticoids, can create an immunosuppressive microenvironment. Other stress-related neurotransmitters, such serotonin (5-hydroxytryptamine, 5-HT), are just now beginning to be understood for their effects on the emergence of cancer.
Qin, Xuan, et al. "Serotonin/HTR1E signaling blocks chronic stress-promoted progression of ovarian cancer." Theranostics 11.14 (2021): 6950.
Pubmed: 34093864 DOI: 10.7150/thno.58956

According to these results, serotonin interaction with the 5-HTR1E receptor in HEK293 cells concurrently activates the cAMP and ERK pathways, and its expression is crucial for cell survival.
Sharma, Vinay Kumar, et al. "Characterization of serotonin-5-HTR1E signaling pathways and its role in cell survival." (2023).
Pubmed: 36789437 DOI: 10.21203/rs.3.rs-2518076/v1