mProX™ Human HTR1D Stable Cell Line

Based on this stable cell line, we also provide cell-based in vitro assays to evaluate the effects of your compounds or antibodies.

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types
S01YF-1122-KX889	Magic™ Human HTR1D in Vitro [35S]GTPγS binding Assay	Human	CHO-K1	[35S]GTPγS binding Assay
S01YF-1122-KX890	Magic™ Human HTR1D in Vitro Radioligand Binding Assay	Human	CHO-K1	Radioligand Binding Assay
S01YF-1122-KX888	Magic™ Dog HTR1D in Vitro Radioligand Binding Assay	Dog	CHO-K1	Radioligand Binding Assay
S01YF-1122-KX891	Magic™ Mouse HTR1D in Vitro Calcium Flux Assay	Mouse	CHO-K1	Calcium Flux Assay
S01YF-1122-KX892	Magic™ Mouse HTR1D in Vitro Radioligand Binding Assay	Mouse	CHO-K1	Radioligand Binding Assay
S01YF-1122-KX893	Magic™ Rabbit HTR1D in Vitro Radioligand Binding Assay	Rabbit	CHO-K1	Radioligand Binding Assay
S01YF-1122-KX894	Magic™ Rat HTR1D in Vitro Calcium Flux Assay	Rat	CHO-K1	Calcium Flux Assay
S01YF-1122-KX895	Magic™ Rat HTR1D in Vitro Radioligand Binding Assay	Rat	CHO-K1	Radioligand Binding Assay

Product Information

Target Protein

HTR1D

Target Family

5-Hydroxytryptamine Family

Target Protein Species

Human

Host Cell Type

HPDE6-C7; CHO-K1; HEK293

Target Classification

GPCR Cell Lines

Target Research Area

CNS Research

Related Diseases

Migraine

Gene ID

3352

UniProt ID

3352

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Due to the presence of HTR1D in human trigeminal ganglia, neurogenic inflammation and nociceptive activity within the trigeminovascular afferents may be HTR1D mediated. HTR1D undergoes a conformational change upon interaction with its ligand, 5-hydroxytryptamine, which initiates signaling via guanine nucleotide-binding proteins and controls the activity of downstream effectors. Adenylate cyclase activity could be inhibited by HTR1D signaling. Specifically through controlling the production of 5-hydroxytryptamine in the brain and consequently affecting neuronal activity, HTR1D operates on the central nervous system and influences movement and anxiety. HTR1D are involved in controlling the release of various neurotransmitters. HTR1D may be implicated in vasoconstriction, according to several investigations. Additionally, it serves as a receptor for several anxiolytic, antidepressant, and hallucinogenic medicines, as well as derivatives of the ergot alkaloid. The customized HTR1D stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Antoine (Verified Customer)

What is the BSL of the HTR1D cell line? Dec 29 2022

chat Sherry Smith (Creative Biolabs Scientific Support)

The BSL of the HTR1D cell line is Level 1. Dec 29 2022

chat Duncan (Verified Customer)

What assays can the HTR1D Stable Cell Line be used for? May 26 2023

chat Sherry Smith (Creative Biolabs Scientific Support)

This cell line is customized for drug discovery, and it also can be used in ELISA and Western Blots. May 26 2023

Published Data

Fig.1 HTR1D promotes proliferation and migration of PCs in vitro.

Compared to the control group, reduced HTR1D had a lower OD value at 450 nm, according to the CCK-8 assay results. According to EdU data, HTR1D knockdown could drastically lower the EdU fluorescence ratio.

Ref: Wu, Wu, et al. "HTR1D functions as a key target of HOXA10-AS/miR-340-3p axis to promote the malignant outcome of pancreatic cancer via PI3K-AKT signaling pathway." International Journal of Biological Sciences 18.9 (2022): 3777.

Pubmed: 35813473

DOI: 10.7150/ijbs.70546

Research Highlights

Colon adenocarcinoma (COAD) is the most prevalent kind of colorectal cancer (CRC), a tumor that arises from the colon or rectum. The goal of the current study was to find the genes that are essential for COAD patients' survival.
Zeng, Chunyan, and Youxiang Chen. "HTR1D, TIMP1, SERPINE1, MMP3 and CNR2 affect the survival of patients with colon adenocarcinoma." Oncology letters 18.3 (2019): 2448-2454.
Pubmed: 31452735 DOI: 10.3892/ol.2019.10545

These results demonstrated that HTR1D has a strong oncogenic impact on GC and may offer a fresh therapeutic target.
Jin, Xinghan, et al. "Knockdown and inhibition of hydroxytryptamine receptor 1D suppress proliferation and migration of gastric cancer cells." Biochemical and biophysical research communications 620 (2022): 143-149.
Pubmed: 35785570 DOI: 10.1016/j.bbrc.2022.06.088