mProX™ Human HRH4 Stable Cell Line

Product Information

Target Protein

HRH4

Target Family

Histamine Family

Target Protein Species

Human

Host Cell Type

ARPE-19;CHO-K1;HEK293

Target Classification

GPCR Cell Lines

Target Research Area

CNS Research

Related Diseases

Meniere Disease;Paraneoplastic Cerebellar Degeneration

Gene ID

Human: 59340

UniProt ID

Human: Q9H3N8

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Histamine receptor H4 (HRH4) has been the subject of various studies, especially in the context of asthma. Polymorphisms in HRH4 have been associated with asthma pathogenesis, suggesting its potential role in the development of the disease. Additionally, the attenuated expression of HRH4 in colorectal carcinomas has been linked to tumor growth and progression, indicating its significance in cancer research.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat David (Verified Customer)

How is HRH4 involved in membrane protein research? Mar 10 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

HRH4, though not directly mentioned in the articles, is likely to be a significant aspect of membrane protein research, similar to other histamine receptors. It could be involved in various cellular processes and responses, potentially playing a role in cell growth and immune responses. Mar 10 2020

chat Lisa (Verified Customer)

Are there any specific studies or methods focusing on HRH4 in cell line development? Sep 26 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

While the articles did not provide direct information on HRH4, methods like Tag-on-Demand, which exploits amber codon suppression technology, can be instrumental in the enrichment of high-expressing membrane protein cell lines, including those involving HRH4. Sep 26 2020

Published Data

Fig.1 Role of HRH4 in modulating the expression of pro-angiogenic and anti-angiogenic factors in ARPE-19 cells.

Displayed are VEGF and IL-6 levels in ARPE-19 cell-conditioned media, assessed through ELISA. The experiments were conducted in triplicate, with error bars depicting the mean±SEM. *P<0.05, **p<0.01. Abbreviations: GAPDH - glyceraldehyde 3-phosphate dehydrogenase, HG - high glucose, HRH4 - histamine receptor H4, IL-6 - interleukin 6, NS - not significant, OC - osmotically controlled, PEDF - pigment epithelium-derived factor, VEGF - vascular endothelial growth factor.

Ref: Lee, Byung Joo, et al. "Histamine causes an imbalance between pro-angiogenic and anti-angiogenic factors in the retinal pigment epithelium of diabetic retina via H4 receptor/p38 MAPK axis." BMJ Open Diabetes Research and Care 8.2 (2020): e001710.

Pubmed: 33328159

DOI: 10.1136/bmjdrc-2020-001710

Research Highlights

Liu L, et al. "Transcriptional analyses provide novel insights into the transgenerational ." Ecotoxicology and environmental safety, 2022.
Poly (I:C), a macromolecule containing double-stranded RNA, is often used to mimic viral infections. Previous research conducted by the authors found that maternal exposure to Poly (I:C) influenced the reproductive abilities of laying hens and their offspring. To better understand the underlying mechanisms, the authors sequenced and analyzed splenic transcriptomes in two groups (Poly (I:C) treatment as the challenged group and saline treatment as the control) across three generations (F0 hens, F1 and F2 offspring). The results revealed that Poly (I:C) maternal stimulation impacted gene expression in both the treated F0 hens and unchallenged subsequent generations. Gene Ontology analysis showed that several biological processes related to immunity were significantly affected in F0 and F1 generations. Moreover, Poly (I:C) maternal stimulation also affected pathways associated with growth, development, biosynthesis, and metabolism in F2 offspring. Correlation analysis revealed that certain differentially expressed genes (DEGs), including PHLDA2 and PODN, showed a potential inheritable effect and were highly correlated with reproductive traits in F1 hens. These findings highlight the far-reaching effects of Poly (I:C) on the transcriptome and provide important insights into maternal stimulation and environmental impacts on multi-generational transmission in poultry.
Pubmed: 36288637 DOI: 10.1016/j.ecoenv.2022.114216