mProX™ Human HRH1 Stable Cell Line

Yao Y, et al. "The Roles of Histamine Receptor 1 (hrh1) in Neurotransmitter System Regulation, ." Molecular neurobiology, 2023.
The important physiological processes mediated by histamine receptors, including their involvement in the pathophysiology of various brain disorders, have been researched. A specific histamine receptor, HRH1, has been linked to the development of neurotransmitter systems and neurogenesis. Altered HRH1 binding and expression have been observed in individuals with schizophrenia, depression, and autism. The researchers aimed to investigate the role of hrh1 in zebrafish development and neurotransmitter system control by creating hrh1(-/-) fish through the CRISPR/Cas9 system. They used multiple methods, such as quantitative PCR, in situ hybridization, and immunocytochemistry, to study neurotransmitter systems and essential brain development genes. Behavioral assessments were also conducted to examine the impact of the histamine receptor on larval and adult fish behavior. The results showed that hrh1(-/-) larvae displayed normal behavior in comparison to hrh1(+/+) siblings. However, they exhibited transient abnormalities in the expression of neurodevelopmental markers and a decrease in tyrosine hydroxylase 1 (Th1)-positive cells, th1 mRNA, and hypocretin (hcrt)-positive cells. These abnormalities were not observed in adult fish. In conclusion, the researchers confirmed that zebrafish lacking hrh1 experience deficits in the dopaminergic and hypocretin systems during early development, but these are compensated for by adulthood. However, adult fish showed impaired sociability and anxious-like behavior, along with downregulation of choline O-acetyltransferase a and LIM homeodomain transcription factor Islet1.
Pubmed: 37474883   DOI: 10.1007/s12035-023-03447-z

Bian S, et al. "Integrative single-cell multiomics analyses dissect molecular signatures of ." National science review, 2023.
In this study, researchers examined the multiomic molecular signatures of human gastric cancer, a highly fatal disease with unclear underlying mechanisms. Multiple samples were taken and analyzed using single-cell multiomics sequencing to identify common transcriptomic alterations, such as down-regulation of genes related to normal stomach function and up-regulation of KRT7, PI3, S100A4, and others. Additionally, aberrant up-regulation of genes typically expressed in normal colorectal epithelial cells was found, possibly influenced by chromatin accessibility and DNA methylation levels. The single-cell DNA methylome landscape was also revealed, with potential biomarkers identified. The study also explored the relationship between genetic lineages, DNA methylation, and transcriptomic clusters at the single-cell level. Diverse levels of DNA methylation were observed among different genetic lineages, with poorer differentiation states associated with higher methylation levels in cancer cells within the same patient. These cells also showed decreased expression of MUC1 and immune-related pathways, as well as limited infiltration of CD8(+) T cells. Through this comprehensive approach, the study sheds light on the molecular signatures and intratumoral heterogeneities of human gastric cancer, providing insights into its differentiation states.
Pubmed: 37347037   DOI: 10.1093/nsr/nwad094