mProX™ Human HCN3 Stable Cell Line

Product Information

Target Protein

HCN3

Target Family

Hyperpolarization-activated, Cyclic Nucleotide-gated Channels

Target Protein Species

Human

Host Cell Type

PC12; CHO-K1; HEK293

Accession Number

NM_020897

Target Classification

Ion Channel Cell Lines

Target Research Area

Cardiovascular Research; CNS Research

Related Diseases

Pyruvate Kinase Deficiency Of Red Cells and Bladder Neck Obstruction

Gene ID

57657

UniProt ID

Q9P1Z3

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Humans' HCN3 gene encodes the protein known as potassium/sodium hyperpolarization-activated cyclic nucleotide-gated ion channel 3. Although the channel's activation by hyperpolarization suggests the funny channels in the heart's sinoatrial node-which are the source of spontaneous electrical rhythm generation-its exact function is unknown. Blocking the gene is linked to the resolution of neuropathic pain episodes, and these channels have recently been linked to chronic pain. The customized HCN3 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Helen

The HCN3 cell line was delivered promptly, and it performed exceptionally well in my assays. Sep 13 2022

chat Verified Customer

chat Michelle

The HCN3 cell line is easy to handle and has shown consistent performance in various experimental setups. Mar 01 2021

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FAQ

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Published Data

Fig.1 Expressions of HCN channel isoforms in PC12 cells with or without NGF treatment.

NGF-treated PC12 cells were used to measure the mRNA and protein expressions of HCN channel isoforms using real-time PCR, western blotting, and immunofluorescence. The results of immunofluorescence clearly show that the HCN1-4 channels' positive signals, or green fluorescence, are mostly located on the membranes.

Ref: Zhong, Li-Ying, et al. "Hyperpolarization-activated cyclic nucleotide-gated ion (HCN) channels regulate PC12 cell Differentiation toward sympathetic neuron." Frontiers in Cellular Neuroscience 13 (2019): 415.

Pubmed: 31616252

DOI: 10.3389/fncel.2019.00415

Research Highlights

The aim of this work was to examine the functional functions and differences in long non-coding RNAs (lncRNAs) expression in neuroblastoma tissue.
Tang, Weitao, et al. "MEG3, HCN3 and linc01105 influence the proliferation and apoptosis of neuroblastoma cells via the HIF-1α and p53 pathways." Scientific reports 6.1 (2016): 36268.
Pubmed: 27824082 DOI: 10.1038/srep36268

In the nervous system, hyperpolarization-activated cyclic nucleotide-gated channels (HCNs) are involved in a number of neuronal processes, such as memory and learning, pain modulation, and vigilance state regulation. It has been demonstrated that human disorders such epilepsy, depression, schizophrenia, and Parkinson's disease are caused by malfunctions or genetic loss of these channels.
Stieglitz, Marc S., et al. "Disturbed processing of contextual information in HCN3 channel deficient mice." Frontiers in molecular neuroscience 10 (2018): 436.
Pubmed: 29375299 DOI: 10.3389/fnmol.2017.00436