mProX™ Human HCN2 Stable Cell Line

Product Information

Target Protein

HCN2

Target Family

Hyperpolarization-activated, Cyclic Nucleotide-gated Channels

Target Protein Species

Human

Host Cell Type

PC12; CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

Cardiovascular Research; CNS Research

Related Diseases

Epilepsy; Idiopathic Generalized; Febrile Seizures

Gene ID

610

UniProt ID

Q9UL51

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Humans' HCN2 gene codes for a protein known as potassium/sodium hyperpolarization-activated cyclic nucleotide-gated ion channel 2. Although the channel's activation by hyperpolarization suggests the funny channels in the heart's sinoatrial node-which are the source of spontaneous electrical rhythm generation-its exact function is unknown. Blocking the gene is linked to the resolution of neuropathic pain episodes, and these channels have recently been linked to chronic pain. The customized HCN2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Richard

The HCN2 cell line worked as advertised and was a cost-effective alternative in our studies. We obtained consistent results, and the cell line showed good stability over extended periods. Dec 02 2022

chat Verified Customer

chat Timothy

The HCN2 cell line worked flawlessly, and the detailed protocols provided by the company made the whole process much easier. Oct 26 2021

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 AAV-mediated shRNA knockdown of HCN2 in the VTA.

Under low and high magnification, immunofluorescence labeling demonstrates the expression of AAV2-HCN2-shRNA-eGFP (green), TH (dopamine neuron marker, red), and NeuN (neuronal marker, blue) in the midbrain.

Ref: Zhong, Peng, et al. "HCN2 channels in the ventral tegmental area regulate behavioral responses to chronic stress." Elife 7 (2018): e32420.

Pubmed: 29256865

DOI: 10.7554/eLife.32420

Research Highlights

Similar to a "pacemaker for pain," the HCN2 isoform functions in nociceptive neurons by maintaining electrical activity, which is necessary for the perception of persistent pain in pathological pain conditions.
Tsantoulas, Christoforos, Elizabeth R. Mooney, and Peter A. McNaughton. "HCN2 ion channels: basic science opens up possibilities for therapeutic intervention in neuropathic pain." Biochemical Journal 473.18 (2016): 2717-2736.
Pubmed: 27621481 DOI: 10.1042/BCJ20160287

These findings demonstrate that HCN2 plays a crucial role in controlling VTA dopamine neuronal activity and behaviors associated with depression.
Zhong, Peng, et al. "HCN2 channels in the ventral tegmental area regulate behavioral responses to chronic stress." Elife 7 (2018): e32420.
Pubmed: 29256865 DOI: 10.7554/eLife.32420