mProX™ Human GRIA1 Stable Cell Line

Product Information

Target Protein

GRIA1

Target Family

Glutamate Receptor

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research

Related Diseases

Alacrima; Achalasia; Mental Retardation Syndrome; Depression.

Gene ID

2890

UniProt ID

P42261

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The GRIA1 gene in humans encodes the protein glutamate receptor 1. Alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors include the GRIA1 receptor. Alternative RNA splicing produces flip and flop isoforms in each of the members (GRIA1-4). Each variant encodes a different receptor subunit, with different signal transduction characteristics. The flop isoform is the one that is being shown here. Human GRIA1 mRNA was found to be present in the granule and pyramidal cells of the hippocampus formation by in situ hybridization tests. The primary factor influencing synaptic plasticity is GRIA1 (GluR1). The human frontal brain has a considerable decrease in GluR1 gene expression as one ages. The customized GRIA1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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The GRIA1 cell line has played a pivotal role in our journey of discovery. Its reliable performance and consistent results have propelled our research forward. Sep 19 2021

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I've used the GRIA1 KO cell line in multiple assays, and each time, it has exceeded my expectations. Jan 03 2023

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FAQ

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Published Data

Fig.1 Location of residues in GluA1 affected by GRIA1 variants and effect on receptor expression.

Confocal imaging of GFP-tagged WT and p and membrane reporter mCardinal-farnesyl expressed in HEK293 cells (left pictures).Arg377Ter variant GluA1 (denoted by R377∗) to see patterns of cellular GluA1 distribution. By combining the left and middle photos (right images), the co-location of green and red fluorescence-which appears yellow-indicates the cell surface localization of GFP-tagged GluA1.

Ref: Ismail, Vardha, et al. "Identification and functional evaluation of GRIA1 missense and truncation variants in individuals with ID: An emerging neurodevelopmental syndrome." The American Journal of Human Genetics 109.7 (2022): 1217-1241.

Pubmed: 35675825

DOI: 10.1016/j.ajhg.2022.05.009

Research Highlights

Sleep and disruption of the circadian rhythm are linked to schizophrenia and dysfunction of the glutamate α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor GluA1 subunit, as well as deficiencies in synaptic plasticity.
Ang, Gauri, et al. "Deletion of AMPA receptor GluA1 subunit gene (Gria1) causes circadian rhythm disruption and aberrant responses to environmental cues." Translational Psychiatry 11.1 (2021): 588.
Pubmed: 34782594 DOI: 10.1038/s41398-021-01690-3

Attention, sensory processing, synaptic plasticity, and memory consolidation have all been linked to sleep EEG spindles. Sleep spindle deficiencies have been linked to a variety of neurological and mental conditions in humans, including schizophrenia.
Ang, Gauri, et al. "Absent sleep EEG spindle activity in GluA1 (Gria1) knockout mice: relevance to neuropsychiatric disorders." Translational Psychiatry 8.1 (2018): 154.
Pubmed: 30108203 DOI: 10.1038/s41398-018-0199-2