mProX™ Human GPR75 Stable Cell Line

Product Information

Target Protein

GPR75

Target Family

Orphan Family

Target Protein Species

Human

Host Cell Type

HTLA;CHO-K1;HEK293

Target Classification

GPCR Cell Lines

Gene ID

Human: 10936

UniProt ID

Human: O95800

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

GPR75 has emerged as a promising target in the realm of metabolic research. Recent groundbreaking studies have identified GPR75 variants associated with protection from obesity. This discovery has profound implications for the understanding of obesity's genetic underpinnings and potential therapeutic interventions. Furthermore, GPR75's role in energy metabolism has been highlighted, with studies suggesting that its deficiency can protect against diet-induced obesity and improve glucose homeostasis. The receptor's involvement in the CYP/20-HETE pathway also suggests its potential relevance in hypertension and related cardiovascular diseases.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Ashley (Verified Customer)

What physiological processes involve GPR75? Sep 03 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

GPR75 is a G-protein coupled receptor implicated in several physiological processes. Its specific functions and interactions are subjects of ongoing research. Sep 03 2021

chat Thomas (Verified Customer)

Can GPR75 be a potential therapeutic target? Jun 28 2023

chat Patrick Liam (Creative Biolabs Scientific Support)

Given its involvement in various physiological processes, GPR75 is being studied as a potential therapeutic target for certain conditions. Jun 28 2023

Published Data

Fig.1 The introduction of 20-HETE at a concentration of 1 nM led to a notable elevation in intracellular calcium levels, affecting both HTLA cells that were mock-transfected and those that were transfected with GPR75 receptors.

Investigative experiments involved monitoring calcium levels in HTLA cells that had been transfected with either GPR75 or a mock control. The cells were subjected to various treatments, including exposure to ethanol, 20-HETE at a concentration of 1 nM, and a co-treatment of 20-HETE (1 nM) with AAA, a receptor antagonist for 20-HETE. Subsequently, live calcium traces were recorded for a duration of 2 minutes following the treatments, providing valuable insights into cellular responses.

Ref: Pascale, Jonathan V., et al. "Uncovering the signalling, structure and function of the 20-HETE-GPR75 pairing: Identifying the chemokine CCL5 as a negative regulator of GPR75." British journal of pharmacology 178.18 (2021): 3813-3828.

Pubmed: 33974269

DOI: 10.1111/bph.15525

Research Highlights

D'Addario CA, et al. "Global and endothelial G-protein coupled receptor 75 (GPR75) knockout relaxes ." Vascular pharmacology, 2023.
Pulmonary hypertension (PH) is a complex disease with a poor prognosis and limited treatment options. Higher expression of the orphan G-Protein Coupled Receptor 75 (GPR75) has been observed in leukocytes and pulmonary arterial smooth muscle cells of patients with idiopathic PH, as well as in C57BL/6 mice exposed to hypoxia. It has been hypothesized that GPR75 signaling is crucial in the pathogenesis of PH. To test this hypothesis, global (Gpr75(-/-)) and endothelial cell (EC) GPR75 knockout (EC-Gpr75(-/-)) mice were exposed to hypoxia for 5 weeks. Echocardiograms and right heart catheterizations showed that chronic hypoxia increased right ventricular pressures in wild-type mice but not in Gpr75(-/-) or EC-Gpr75(-/-) mice. Further analysis revealed increased Gpr75 expression in the lungs of hypoxia-exposed mice, particularly in the alveoli, airways, and pulmonary arteries. Additionally, hypoxia-induced levels of chemokine (CC motif) ligand 5 (CCL5) were significantly higher in wild-type mice compared to Gpr75(-/-) mice. Further experiments showed that CCL5 enhanced hypoxia-induced pulmonary artery contractions in a GPR75-dependent manner. Knocking out Gpr75 also increased cAMP levels and decreased pulmonary artery contractions induced by endothelin-1 or U46619, both in a cAMP-protein kinase A-dependent manner. These findings suggest a significant role for GPR75 in the development of hypoxia-induced PH.
Pubmed: 37742819 DOI: 10.1016/j.vph.2023.107235

Choi J, et al. "A whole-genome reference panel of 14,393 individuals for East Asian populations ." Science advances, 2023.
The growth of global precision medicine is hindered by the underrepresentation of non-European (EUR) populations. To address this issue, a team of researchers created a reference panel of 14,393 whole-genome sequences including more than 11,000 individuals of Asian descent. Using this panel, they conducted genome-wide association studies on 72,298 subjects from a specific population, yielding improved accuracy for rare and low-frequency variants within East Asian populations. Through this study, 39 previously unidentified associations were found, including rare protein-altering variants in genes such as LTBP1 for height and GPR75 for body mass index. The team also identified potential regulatory mechanisms for rare noncoding variants with cell type-specific effects. Overall, the resulting dataset could significantly contribute to the advancement of Asian precision medicine.
Pubmed: 37556544 DOI: 10.1126/sciadv.adg6319