mProX™ Human GPR4 Stable Cell Line

Li R, et al. "GPRASP1 loss-of-function links to arteriovenous malformations by endothelial ." Brain : a journal of neurology, 2023.
This study examines the genetic pathogenesis of arteriovenous malformations (AVMs), which are a common cause of intracerebral hemorrhage in young adults. The researchers identified two missense variants of G protein-coupled receptor (GPCR) associated sorting protein 1 (GPRASP1) in AVM patients for the first time. These variants were found to be loss-of-function (LoF) in endothelial cells and resulted in endothelial dysfunction. The researchers also found a link between GPRASP1 and GPR4, another GPCR that is involved in causing endothelial disorders. They discovered that GPRASP1 plays a role in promoting GPR4 degradation, which leads to disturbed endothelial functions and contributes to vascular anomalies. The researchers suggest that targeting GPR4 with specific drugs could potentially help in treating AVMs.
Pubmed: 37787182   DOI: 10.1093/brain/awad335

Shu T, et al. "Effects of short-term water velocity stimulation on the biochemical and ." Frontiers in physiology, 2023.
Since 2011, the ecological operation trials of the Three Gorges Reservoir (TGR) have been ongoing in order to enhance the spawning quantity of the four major Chinese carp species below the Gezhouba Dam. In particular, it is important to investigate the effects of short-term water velocity stimulation on the ovarian development of grass carp (Ctenopharyngodon idellus) in order to understand the response of natural reproduction to ecological flows. Through ovary histology analysis and biochemical assays on individuals under stimulation from running water, it was found that while there were no significant impacts on the ovarian development of grass carp, there were elevated levels of hormones including estradiol, progesterone, follicle-stimulating hormone (FSH), and triiodothyronine (T3). Further examination through RNA sequencing of ovarian tissues revealed that 221 and 741 genes were up- and downregulated, respectively, under short-term water velocity stimulation compared to a control group. The majority of these differentially expressed genes were enriched in pathways such as ABC transporters, cytokine-cytokine receptor interaction, ECM-receptor interaction, and steroid hormone biosynthesis. Important genes involved in ovarian development, including gpr4, vtg1, C-type lectin, hsd17b1, cyp19a1a, cyp17a1, and rdh12, were found to be regulated. These findings offer new insights and suggest potential regulatory genes and pathways involved in the ovarian development of grass carp under short-term water velocity stimulation, which could be beneficial for future ecological regulation strategies.
Pubmed: 37719458   DOI: 10.3389/fphys.2023.1248999