mProX™ Human GPR37 Stable Cell Line

Product Information

Target Protein

GPR37

Target Family

Orphan Family

Target Protein Species

Mouse

Host Cell Type

MC-38;CHO-K1;HEK293

Target Classification

GPCR Cell Lines

Target Research Area

Ocular Research;CNS Research

Related Diseases

Juvenile-Onset Parkinson's Disease;Epilepsy, Familial Temporal Lobe, 6

Gene ID

Mouse: 14763

UniProt ID

Mouse: Q9QY42

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

GPR37, a G protein-coupled receptor, has been associated with various neurological processes. Recent research has highlighted its protective role against infection-induced sepsis and pain-like behavior in mice. Additionally, GPR37's involvement in the progression of lung adenocarcinoma has been documented, suggesting its potential as a therapeutic target. The receptor's role in regulating oligodendrocyte differentiation and myelination in the central nervous system further underscores its significance in neurological health. Moreover, GPR37 has been linked to Parkinson's disease, making it a potential biomarker for this neurodegenerative condition.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Jennifer (Verified Customer)

What is the role of GPR37 in cellular functions? Jun 23 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

GPR37, a G protein-coupled receptor, is implicated in various cellular functions. When properly folded and trafficked to the plasma membrane, it exhibits a protective role against dopaminergic toxins in certain cell types like N2a neuroblastoma cells. Moreover, the subcellular distribution of GPR37 affects cell viability, where its accumulation and aggregation within the cell can cause cell death, while its presence in the plasma membrane provides cell protection. Jun 23 2022

chat Jessica (Verified Customer)

What are the potential research applications of GPR37 custom cell line products? Oct 29 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

GPR37 custom cell line products can serve as invaluable tools in studying the molecular mechanisms underlying GPR37's function and its role in cell viability and protection against certain toxins. These custom cell lines enable researchers to delve into the receptor's interactions with other proteins and molecules, its trafficking to the plasma membrane, and its implication in diseases such as parkinsonism. Additionally, GPR37 custom cell lines can aid in drug discovery efforts aimed at modulating GPR37 activity or preventing its intracellular aggregation, potentially offering therapeutic strategies for related neurological conditions. Oct 29 2021

Published Data

Fig.1 GPR37 depletion suppressed CRC liver metastasis (CRLM) in vivo.

A CRLM mouse model was generated to explore the activities of GPR37 in vivo by introducing GPR37 control or GPR37 silenced CRC mouse cell line MC38 into the spleen of C57BL/6 mice, respectively. When one mouse died, all mice were slaughtered at the same time to determine liver metastases. GPR37 knockdown in MC38 cells effectively decreased liver metastasis compared to control cells in both liver metastasis models, as shown by a considerable reduction in the number of metastatic nodules and liver weight.

Ref: Zhou, Jiamin, et al. "GPR37 promotes colorectal cancer liver metastases by enhancing the glycolysis and histone lactylation via Hippo pathway." Oncogene (2023): 1-12.

Pubmed: 37749229

DOI: 10.1038/s41388-023-02841-0

Research Highlights

Zhou J, et al. "GPR37 promotes colorectal cancer liver metastases by enhancing the glycolysis and ." Oncogene, 2023.
In this study, the researchers discovered a significant increase in GPR37 expression in human colorectal cancer (CRC) liver metastasis specimens, which was associated with poor prognosis. They also found that GPR37 depletion effectively inhibited liver metastasis in mouse models of CRC liver metastasis (CRLM). Functional experiments revealed that GPR37 knockdown reduced the glycolysis of CRC cells, resulting in decreased tumor growth. Additionally, GPR37 knockdown decreased the levels of two chemokines involved in neutrophil accumulation, leading to a decrease in neutrophil recruitment in the tumor microenvironment of CRLM. The researchers also found that GPR37 activated the hippo pathway, promoting the expression of LDHA and glycolysis. This led to increased lactylation of H3K18la, which upregulated the chemokines CXCL1 and CXCL5. These findings suggest that GPR37 plays a role in modulating the tumor metabolism and microenvironment in CRLM and could potentially be a therapeutic target.
Pubmed: 37749229 DOI: 10.1038/s41388-023-02841-0

Liu H, et al. "Activation of PI3K/Akt pathway by G protein-coupled receptor 37 promotes ." Cancer medicine, 2023.
Recent research has highlighted the significance of G protein-coupled receptor 37 (GPR37) in the development and progression of non-small-cell lung cancer (NSCLC). Through various analyses, including data from TCGA, GTEx, GEO, and GEPIA2, the expression and prognostic value of GPR37 in NSCLC were investigated. Results showed that GPR37 is highly expressed in NSCLC tissues and cell lines and is associated with poor prognosis. Moreover, GPR37 was found to play a crucial role in regulating key processes such as cell proliferation, apoptosis, and invasion in NSCLC. Through further experiments and analyses, it was discovered that GPR37 activates specific pathways, such as the PI3K/Akt/mTOR pathway, to promote NSCLC progression through mechanisms such as epithelial-mesenchymal transition (EMT). These findings suggest that GPR37 may serve as a potential therapeutic target for the treatment of NSCLC.
Pubmed: 37732632 DOI: 10.1002/cam4.6543