mProX™ Human GPR25 Stable Cell Line

Chen Z, et al. "IL-12RB1: a novel immune prognostic biomarker for oral squamous cell carcinoma ." Annals of translational medicine, 2022.
This study aimed to screen and identify potential immune biomarkers for predicting the prognosis of oral squamous cell carcinoma (OSCC). Data from the Cancer Genome Atlas (TCGA) database was used to calculate stromal and immune scores using the ESTIMATE algorithm for OSCC patients. Differentially expressed genes (DEGs) between high and low immune score groups were identified and validated. The expression of programmed cell death 1 (PD-1)/programmed death-ligand 1 (PD-L1) was evaluated in relation to DEGs through correlation analysis. The expression profiles of three candidate genes were studied using single-cell RNA sequencing in OSCC patients. Finally, immunohistochemistry (IHC) and immunofluorescence (IF) were applied to confirm the expression pattern of interleukin 12 receptor subunit beta 1 (IL-12RB1) in OSCC tissue microarray. The high immune score group showed better overall survival (OS) compared to the low immune score group. Among 339 DEGs, 90 were identified as being closely associated with OS. In the validation set, 23 genes were confirmed to be significantly associated with survival prognosis, and the expression levels of IL-12RB1, cytotoxic T-lymphocyte associated protein 4 (CTLA4), and G protein-coupled receptor 25 (GPR25) were correlated with PD-1/PD-L1 expression. IL-12RB1 was expressed in both epithelial and immune cells as observed through RNA-sequencing and IHC staining, whereas CTLA4 and GPR25 were poorly expressed in OSCC tissue. IF showed co-expression of IL-12RB1 with CD3, CD68, PD-1, and PD-L1 on the cytomembrane. Additionally, high expression of IL-12RB1 in non-malignant cells was found to be a prognostic risk factor for OS in OSCC patients. In conclusion, IL-12RB1 was closely associated with survival in OSCC and with the expression levels of PD-1/PD-L1 in the tumor immune microenvironment.
Pubmed: 35284546   DOI: 10.21037/atm-21-6915

Zhang J, et al. "The orphan G protein-coupled receptor 25 (GPR25) is activated by Apelin and Apela ." Biochemical and biophysical research communications, 2018.
G protein-coupled receptor 25 (GPR25) is an orphan receptor found in vertebrates that has been linked to autoimmune diseases and the regulation of blood pressure. Despite its potential impact, the endogenous ligand for GPR25 in vertebrates remains unknown. Recent research has shown that in non-mammalian vertebrates, such as zebrafish, spotted gars, and pigeons, GPR25 can be activated by Apelin and Apela peptides, which are also the endogenous ligands for the vertebrate Apelin receptor (APLNR). This was demonstrated through the use of a pGL3-CRE-luciferase reporter assay and confocal microscopy, which showed that zebrafish GPR25 expressed in HEK293 cells can effectively bind to Apelin and Apela, leading to a decrease in cAMP production and receptor internalization. Similar results were found in spotted gar and pigeon GPR25, suggesting a conserved function across non-mammalian species. Interestingly, human GPR25 was not activated by these peptides under the same experimental conditions. Further RNA-seq analysis revealed that GPR25 is expressed in a variety of tissues, including the testes and intestine of zebrafish, spotted gars, and humans, indicating a potential broad role for GPR25 signaling in many physiological processes in vertebrates. This study provides the first evidence that GPR25 can be activated by Apelin and Apela in non-mammalian vertebrates, shedding light on the potential physiological functions of this orphan receptor in vertebrates.
Pubmed: 29727602   DOI: 10.1016/j.bbrc.2018.04.229