mProX™ Human GPR20 Stable Cell Line

George S, et al. "A Phase I, Multicenter, Open-Label, First-in-Human Study of DS-6157a in Patients ." Clinical cancer research : an official journal of the American Association for , 2023.
DS-6157a, an antibody-drug conjugate targeting G protein-coupled receptor 20 (GPR20), was evaluated in patients with gastrointestinal stromal tumors (GIST) in a phase I multicenter, open-label, multiple-dose study. The primary objective was to assess the safety and tolerability of DS-6157a and determine the maximum tolerated dose (MTD). Secondary objectives included examining plasma pharmacokinetics, antidrug antibodies (ADA), and efficacy. Thirty-four patients were enrolled, and DS-6157a was found to be well-tolerated with DLTs observed in 4 patients (11.8%) at doses of 6.4 mg/kg, 9.6 mg/kg, and 12.8 mg/kg. The MTD was determined to be 6.4 mg/kg. Treatment-emergent adverse events (TEAE) occurred in 17 patients (50.0%), with four patients (11.8%) experiencing serious adverse events related to DS-6157a. Tumor shrinkage was observed in 20.6% of patients, and 2.9% achieved a partial response. Plasma concentrations of intact DS-6157a, DXd, and total anti-GPR20 antibody showed a dose-dependent profile. No treatment-emergent ADAs were observed. While DS-6157a showed promising results in targeting GPR20 in patients with advanced GIST, it did not proceed further due to lower efficacy outcomes than anticipated.
Pubmed: 37363962   DOI: 10.1158/1078-0432.CCR-23-0640

Persichilli C, et al. "Exploring genome-wide differentiation and signatures of selection in Italian and ." Journal of dairy science, 2023.
In this study, a medium-density genome-wide data set consisting of 79,464 imputed SNPs was used to examine the genetic structure of the Italian Holstein breed, specifically the population reared in the region of Parmigiano Reggiano protected designation of origin cheese production. Multidimensional scaling and ADMIXTURE approaches were employed to investigate the genetic relationships among three Holstein populations: Italian, North American, and Parmigiano Reggiano. Furthermore, four different statistical methods were used to identify putative genomic regions under selection in these populations, including those related to milk quality, resistance to disease, and fertility. The results revealed clear genetic distinctions between the three populations, with the most significant difference being observed between the Italian and North American stock. Additionally, 22 genes related to milk production were identified through allele frequency approaches, with the VPS8 gene showing evidence of convergent selection. Seven genomic regions containing candidate genes for milk traits were also identified through extended haplotype homozygosity analyses. These findings provide important insights into the genetic diversity and selective pressures among these Holstein populations, and may have practical implications for future breeding and selection strategies.
Pubmed: 37291034   DOI: 10.3168/jds.2022-22159