mProX™ Human GPR176 Stable Cell Line

Product Information

Target Protein

GPR176

Target Family

Orphan Family

Target Protein Species

Human

Host Cell Type

DLD-1;HCT116;CHO-K1;HEK293

Target Classification

GPCR Cell Lines

Target Research Area

CNS Research

Related Diseases

Narcolepsy

Gene ID

Human: 11245

UniProt ID

Human: Q14439

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

GPR176 is a G-protein coupled receptor that has garnered attention in the scientific community due to its potential role in the regulation of circadian rhythms. Recent studies have shown that GPR176 might act as a molecular clock in the suprachiasmatic nucleus, the primary circadian pacemaker in mammals. Its involvement in timekeeping mechanisms suggests that GPR176 could play a crucial role in maintaining the body's internal rhythm, influencing processes like sleep, metabolism, and hormone secretion6. Understanding the intricacies of GPR176's function could provide insights into the treatment of circadian rhythm disorders and other related conditions.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat William (Verified Customer)

What is the potential correlation between GPR176 and cancer? Jul 28 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

Research suggests that GPR176 may be a potential target gene in gastric cancer, with implications for immune cell infiltration in the tumor microenvironment. Jul 28 2020

chat Amanda (Verified Customer)

How does GPR176 influence circadian behaviors? Jan 19 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

GPR176 is a Gz-linked orphan G-protein-coupled receptor that has been associated with the regulation of circadian behaviors, acting as a pacemaker to maintain circadian rhythms. Jan 19 2020

Published Data

Fig.1 In vitro experiments revealed that GPR176 actively facilitated cellular proliferation while inducing a halt in the S phase progression.

Flow cytometry (FCM) was employed to assess cell cycle progression and apoptosis in cells with GPR176 knockdown, specifically DLD-1 and HCT116 cells. The results were expressed as the mean ± standard deviation, with significance denoted as ***p < 0.001, **p < 0.01, and *p < 0.05.

Ref: Tang, Junwei, et al. "GPR176 promotes cancer progression by interacting with G protein GNAS to restrain cell mitophagy in colorectal cancer." Advanced Science 10.12 (2023): 2205627.

Pubmed: 36905238

DOI: 10.1002/advs.202205627

Research Highlights

El-Sayed AM, et al. "The role of antifreeze genes in the tolerance of cold stress in the Nile tilapia ." BMC genomics, 2023.
The present study aimed to investigate the effects of cold stress on the up-regulation of antifreeze protein (AFP) genes in Nile tilapia (Oreochromis niloticus). Two treatment groups of fish were investigated (5 replicates of 15 fish for each group in fibreglass tanks/70 L each): a control group and a cold stress group. The immune response of fish exposed to cold stress was detected and characterized using Differential Display-PCR (DD-PCR). The results indicated that nine different up-regulation genes were detected in the cold-stressed fish compared to the control group. These genes were identified as Integrin-alpha-2 (ITGA-2), Gap junction gamma-1 protein-like (GJC1), WD repeat-containing protein 59 isoform X2 (WDRP59), NUAK family SNF1-like kinase, G-protein coupled receptor-176 (GPR-176), Actin cytoskeleton-regulatory complex protein pan1-like (PAN-1), Whirlin protein (WHRN), Suppressor of tumorigenicity 7 protein isoform X2 (ST7P) and ATP-binding cassette sub-family A member 1-like isoform X2 (ABCA1). Furthermore, the antifreeze gene type-II was amplified using a specific PCR product of 600 bp, followed by cloning and sequencing analysis. The identified gene was found to be antifreeze type-II, with a similarity ranging from 70 to 95%. Finally, the in-vitro transcribed gene induced an antifreeze protein with a molecular size of 22 kDa. It was determined that the antifreeze gene, ITGA-2 and the WD repeat protein belong to the lectin family (sugar-protein). In conclusion, under cold stress, Nile tilapia express many defence genes, including an antifreeze gene consisting of one open reading frame of approximately 0.6 kbp.
Pubmed: 37612592 DOI: 10.1186/s12864-023-09569-x

Yun WJ, et al. "The promoting effects of GPR176 expression on proliferation, chemoresistance, ." Journal of cancer research and clinical oncology, 2023.
In this study, the impact of the G-protein-coupled receptor 176 (GPR176) gene on oesophageal cancer was evaluated. GPR176 is a glycosylated protein consisting of 515 amino acids and belongs to the G-protein-coupled receptor 1 family. Bioinformatics and clinical tissue samples were used to analyze the expression and clinicopathological significance of GPR176 in oesophageal cancer. The results showed that GPR176 mRNA expression was significantly higher in oesophageal cancer compared to normal mucosa. Additionally, GPR176 knockdown inhibited various phenotypic characteristics of oesophageal cancer cells, including proliferation, migration, invasion, and lipid droplet formation, while ACC1 and ACLY overexpression reversed these effects. These findings suggest that upregulation of GPR176 may play a role in the development, progression, aggressiveness, and chemoresistance of oesophageal cancer through ACC1- and ACLY-mediated lipogenesis and lipid droplet formation.
Pubmed: 37584712 DOI: 10.1007/s00432-023-05256-2