mProX™ Human GPR171 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- GPCR Cell Lines
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Made to Order Inquiry
InquiryProduct Information
Product Properties
Protocols
Please visit our protocols page.
Customer Reviews
There are currently no Customer reviews or questions for mProX™ Human GPR171 Stable Cell Line (S01YF-0923-PY177). Click the button above to contact us or submit your feedback about this product.
Kimberly (Verified Customer)
Patrick Liam (Creative Biolabs Scientific Support)
Michael (Verified Customer)
Patrick Liam (Creative Biolabs Scientific Support)
Published Data
Fig.1 When GPR171 expression is diminished or obstructed, it leads to a notable attenuation in the signal transmission mediated by BigLEN.
The heightened expression of GPR171 results in a substantial elevation in specific [125I]Tyr-BigLEN binding within Neuro2A cells, while the introduction of shRNA targeting GPR171 leads to a marked reduction in binding compared to Neuro2A cells (p < 0.0001).
Ref: Gomes, Ivone, et al. "GPR171 is a hypothalamic G protein-coupled receptor for BigLEN, a neuropeptide involved in feeding." Proceedings of the National Academy of Sciences 110.40 (2013): 16211-16216.
Pubmed: 24043826
DOI: 10.1073/pnas.1312938110
Research Highlights
Baalmann F, et al. "Reduced urine volume and changed renal sphingolipid metabolism in ." Frontiers in cell and developmental biology, 2023.
The UDP-glucose receptor P2RY14, a rhodopsin-like G protein-coupled receptor (GPCR), has been previously described as a receptor expressed in A-intercalated cells of the mouse kidney. Recent studies have shown that P2RY14 is also abundantly expressed in the collecting duct principal cells of the papilla and the epithelial cells lining the renal papilla. To gain a better understanding of its role in the kidney, a P2ry14 reporter and gene-deficient (KO) mouse strain were utilized. Morphometric analysis revealed that P2RY14 contributes to kidney morphology, as KO mice had a broader cortex compared to wild-type (WT) mice. In addition, the outer stripe of the outer medulla was larger in WT mice than KO mice. Transcriptome comparison of WT and KO mice showed differences in gene expression, including extracellular matrix proteins and proteins involved in sphingolipid metabolism and other GPCRs. Mass spectrometry analysis revealed changes in sphingolipid composition in the renal papilla of KO mice. At the functional level, KO mice had a reduced urine volume but an unchanged glomerular filtration rate under normal chow and salt diets. This study highlights the importance of P2RY14 in the collecting duct principal cells and cells lining the renal papilla, and suggests a potential role in nephroprotection through the regulation of decorin.
Pubmed:
37250906
DOI:
10.3389/fcell.2023.1128456
McDermott MV, et al. "A small molecule ligand for the novel pain target, GPR171, produces minimal ." Pharmacology, biochemistry, and behavior, 2023.
The drug MS15203, which activates the receptor GPR171, has been demonstrated to enhance the effectiveness of morphine in relieving pain. Through the use of in vivo and histological methods, the authors have shown that MS15203 does not activate the reward circuitry in rodents, further supporting its potential as a novel pain medication and GPR171 as a potential pain target. These findings suggest that more research should be conducted on MS15203 for pain management.
Pubmed:
36933620
DOI:
10.1016/j.pbb.2023.173543