mProX™ Human GPR157 Stable Cell Line

Wang S, et al. "Histopathological and transcriptomic analyses reveal the reproductive ." The Science of the total environment, 2023.
The novel brominated flame retardant DBDPE has emerged as a common environmental contaminant and has been linked to potential disruptions of the reproductive endocrine system in vertebrates. The effects of DBDPE on invertebrates, however, remain largely unknown. In this study, researchers exposed mussels of the species Mytilus galloprovincialis to varying concentrations of DBDPE for a period of 30 days. A combination of histopathological and transcriptomic analyses was used to evaluate the reproductive endocrine-disrupting effects of DBDPE and the underlying mechanisms. Results showed that DBDPE promoted gametogenesis in both male and female mussels as evidenced by histological observations, gender-specific gene expression (VERL and VCL), and morphometric data. Transcriptomic analysis further revealed that DBDPE interfered with cholesterol homeostasis and transport through the actions of different LRPs and ABCs, as well as disrupted nongenomic signaling pathways such as GPR157-IP3-Ca(2+) in males and cGMP in females, leading to perturbations in the expression of reproductive genes (VMO1, ZAN, Banf1, and Hook1). Additionally, DBDPE was found to influence energy metabolism in male mussels, potentially influencing the reproductive endocrine system. This is the first study to report on the reproductive endocrine-disrupting effects of DBDPE in marine mussels, providing valuable insights for the ecological risk assessment of DBDPE pollution in marine environments.
Pubmed: 36493811   DOI: 10.1016/j.scitotenv.2022.160724

Takeo Y, et al. "The G protein-coupled receptor GPR157 regulates neuronal differentiation of ." Scientific reports, 2016.
In this study, the researchers investigated the role of the orphan G protein-coupled receptor GPR157 in the ability of radial glial progenitors (RGPs) to generate cortical neurons. It was found that GPR157 localizes to RGPs' primary cilia exposed to the cerebrospinal fluid (CSF) and signals through the Gq-class of heterotrimeric G-proteins via IP3-mediated Ca(2+) cascade. Activation of GPR157-Gq signaling promotes neuronal differentiation of RGPs, while interfering with this cascade suppresses neurogenesis. The CSF was also found to contain putative ligands for GPR157, which enhance its ability to activate GPR157 at the neurogenic phase. Hence, the activation of GPR157-Gq signaling at the primary cilia of RGPs by the CSF plays a crucial role in neurogenesis. The findings suggest that local extracellular factors and intrinsic signaling pathways of RGPs, such as GPR157-Gq, determine their ability to generate cortical neurons.
Pubmed: 27142930   DOI: 10.1038/srep25180