mProX™ Human GPR150 Stable Cell Line

Product Information

Target Family

Orphan Family

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1

Target Classification

Other Targets Drug Discovery Assays and Products

Gene ID

Human:285601

UniProt ID

Human:Q8NGU9

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

GPR150, although less studied than some of its counterparts, has been identified in the context of ovarian cancers. Specifically, aberrant methylation of GPR150 has been observed in ovarian cancers, suggesting its potential role as a tumor marker. As research on GPR150 continues, it is anticipated that its functions and implications in health and disease will become clearer.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Shirley (Verified Customer)

What is the role of GPR150 in ovarian cancers? Apr 03 2023

chat Patrick Liam (Creative Biolabs Scientific Support)

GPR150 is associated with aberrant promoter methylation in ovarian cancers. It is one of the genes that can be methylated in ovarian cancer cell lines, indicating a potential role in the disease's development or progression. Apr 03 2023

chat Stephanie (Verified Customer)

Is GPR150 involved in any cellular processes or pathways? Oct 20 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

Specific cellular roles of GPR150 are not well-defined yet. However, its classification as a GPCR suggests involvement in cellular signaling pathways. Further research is needed to elucidate its specific functions and implications in health and disease. Oct 20 2021

Published Data

Fig.1 GPR150 expression levels in normal and malignant cell lines.

GPR150 expression levels in normal and ten cancer cell lines. Quantitative RT-PCR was used to examine expression levels. Sample 1: HOSE6-3; samples 2-11: 10 ovarian cell lines (ES-2, TYK-nu, TOV-21G, MCAS, RMG-I, TOV-112D, OV-90, RTSG, RUMG-L, and KURAMOCHI); sample 13: normal brain. The letters M, B, and U indicate the presence of exclusively methylated DNA, both methylated and unmethylated DNA, and only methylated DNA, respectively.

Ref: Cai, Li-yi, et al. "Identification of PRTFDC1 silencing and aberrant promoter methylation of GPR150, ITGA8 and HOXD11 in ovarian cancers." Life sciences 80.16 (2007): 1458-1465.

Pubmed: 17303177

DOI: 10.1016/j.lfs.2007.01.015

Research Highlights

Danial Hashemi Karoii et al. "Altered G-Protein Transduction Protein Gene Expression in the Testis of Infertile Patients with Nonobstructive Azoospermia." DNA and cell biology, 2023
Recent studies have shown that members of the G-protein-coupled receptor (GPCR) superfamily play crucial roles in maintaining ion-water homeostasis in sperm and Sertoli cells, as well as in germ cell development, blood barrier formation, and sperm maturation. Through microarray and bioinformatics analysis of 3513 sperm and Sertoli cell genes, researchers have identified differentially expressed genes (DEGs) involved in these processes. Specifically, in samples from cases of nonobstructive azoospermia (NOA), gene expression of GOLGA8IP, OR2AT4, PHKA1, A2M, and others was upregulated, while expression of MARS, SIRPG, OGFR, and others was downregulated. Sertoli cells in NOA cases also showed changes in gene expression, including upregulation of GBP3, TNF, and others. Functional enrichment analysis using Enrich Shiny Gene Ontology (GO), STRING, and Cytoscape predicted interactions among these proteins and identified important molecular pathways. GO Enrichment also showed that genes involved in regulating protein metabolic processes and small GTPase-mediated signaling were significantly differentially expressed in sperm samples. Furthermore, gene mutation analysis showed common BP and MF in these DEGs between sperm and Sertoli cells. These findings could potentially lead to the development of GPCR-targeted therapeutic options.
Danial Hashemi Karoii et al. "Altered G-Protein Transduction Protein Gene Expression in the Testis of Infertile Patients with Nonobstructive Azoospermia." DNA and cell biology, 2023
Pubmed: 37610843 DOI: 10.1089/dna.2023.0189

Guohui Bai et al. "Systematic analysis of differentially methylated expressed genes and site-speciﬁc methylation as potential prognostic markers in head and neck cancer." Journal of cellular physiology, 2019
The study focused on the role of DNA methylation in predicting the prognosis of head and neck cancer (HNC). Using gene expression and methylation data from the Cancer Genome Atlas database, the researchers utilized bioinformatics analysis to identify abnormal differential methylation genes (DMGs) and pathways in head-neck squamous cell carcinoma. Five DMGs were found to be significantly correlated with overall survival and were used to construct a prognostic model. The model was able to predict 5-year survival with an area under the curve of 0.665. Additionally, the correlation between DMGs, site-specific methylation, and gene expression was studied. The results suggest that these five DMGs can serve as independent prognostic biomarkers for HNC, potentially aiding in early diagnosis.
Guohui Bai et al. "Systematic analysis of differentially methylated expressed genes and site-speciﬁc methylation as potential prognostic markers in head and neck cancer." Journal of cellular physiology, 2019
Pubmed: 31131446 DOI: 10.1002/jcp.28835