mProX™ Human GPR142 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- GPCR Cell Lines
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Published Data
Fig.1 The impact of Gpr142 agonists on glucose-triggered insulin release was studied in both control and Gpr142-KD INS-1832/13 cell lines.
In a series of experiments, INS-1832/13 cells underwent incubation in two different glucose concentrations: 1 mM glucose and 16.7 mM glucose. This incubation was performed in the presence of either compound 33 or compound A, each at a concentration of 1 μM, for a duration of 60 minutes. Subsequently, insulin secretion was assessed, and the results are presented as means ± SEM from four independent experiments, with 2-3 replicates within each experimental group. Significant differences are denoted by *p < 0.05, **p < 0.01, and ***p < 0.001.
Ref: Al-Amily, Israa Mohammad, et al. "The functional impact of G protein-coupled receptor 142 (Gpr142) on pancreatic β-cell in rodent." Pflügers Archiv-European Journal of Physiology 471 (2019): 633-645.
Pubmed: 30767071
DOI: 10.1007/s00424-019-02262-7
Research Highlights
Zhao B, et al. "The fiber diameter traits of Tibetan cashmere goats are governed by the inherent ." BMC genomics, 2022.
Tibetan cashmere goats are an important model for studying high altitude adaptation and hypoxia complications. The cashmere produced by these goats is a vital source of income for the herders. The aim of this study was to investigate the differences in protein abundance between fine and coarse cashmere produced by Tibetan cashmere goats. A label-free proteomics approach was used to identify 1980 proteins, of which 1730 were mapped to the original protein coding genes. Comparative analysis of cashmere with fine and coarse phenotypes revealed 29 differentially expressed proteins, including GC, VTN, AEBP1, and GPR142. Functional enrichment analysis showed these proteins were involved in important pathways such as oxidation-reduction and cell redox homeostasis. Additionally, transcription factors enrichment analysis revealed that the proteins mainly belonged to NF-YB, HMG, and CSD families. Western blot validation of four of these differentially expressed proteins suggested they could be potential candidate genes for cashmere traits in Tibetan cashmere goats. These findings shed light on the genetic and biological mechanisms underlying cashmere production and can inform breeding strategies for cashmere traits in these goats.
Pubmed:
35255833
DOI:
10.1186/s12864-022-08422-x
Osuga Y, et al. "Identification of a regulatory pathway of L-phenylalanine-induced GLP-1 secretion ." Biochemical and biophysical research communications, 2022.
GLP-1, an incretin hormone, is released by enteroendocrine L cells upon detection of saccharides, amino acids, and fatty acids. It has been observed that certain amino acids, such as L-phenylalanine, stimulate GLP-1 secretion in these cells. However, the precise mechanism by which L-phenylalanine triggers GLP-1 secretion is still unknown. Thus, the authors performed live-cell imaging experiments to investigate the pathway by which L-phenylalanine activates enteroendocrine L cells. The findings suggest that L-phenylalanine stimulates GLP-1 secretion by binding to GPR142, a G(q)-coupled receptor, and entering the cell through a Na(+)-dependent amino acid transporter, resulting in an increase in intracellular Ca(2+) concentration and membrane depolarization. Overall, the study provides insights into the potential role of enteroendocrine L cells in regulating blood glucose levels via the sensing and secretion of GLP-1 in response to L-phenylalanine through the GPR142 and Na(+)-dependent amino acid transporters.
Pubmed:
34953208
DOI:
10.1016/j.bbrc.2021.12.043
