mProX™ Human GPR12 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- GPCR Cell Lines
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Published Data
Fig.1 GPR12 initiates a significant increase of Ki67 to promote cell proliferation.
In HEK293 cells with overexpressed GPR12 tagged with myc (highlighted in red), immunostaining was conducted using an anti-myc tag antibody, followed by a Cy3-labeled secondary antibody. The cellular localization was visualized alongside Ki67 (in green) and DAPI (in blue) staining, with a 50 µm scale bar provided for reference. Co-immunostaining of HEK293 cells transfected with GPR12 and those with the vector was performed using DAPI stain (blue dye) and an anti-Ki67 antibody (red dye) to assess cell proliferation.
Ref: Lu, Xiaoming, et al. "Involvement of GPR12 in the regulation of cell proliferation and survival." Molecular and cellular biochemistry 366 (2012): 101-110.
Pubmed: 22430950
DOI: 10.1007/s11010-012-1287-x
Research Highlights
Pan G, et al. "Exosomal miR-105-5p derived from bladder cancer stem cells targets for GPR12 to ." BMC urology, 2023.
Bladder cancer stem cells (BCSCs) have been identified as the causative factor for initiating and recurring cases of bladder cancer. Exosomes derived from BCSCs (CSCs-exo) play a crucial role in creating a stable tumor microenvironment. While miR-105-5p has been found to promote tumor growth in various cancers, its effects on bladder cancer have not been thoroughly investigated. The characteristics of CSCs-exo were evaluated using transmission electron microscopy and nanoparticle tracking analysis. The cellular uptake of exosomes was observed using the PKH67 dye. Various assays were performed to assess cell viability, migration, and invasion. The interaction between miR-105-5p and GPR12 was confirmed through a luciferase activity assay. Xenografts were induced in nude mice and histological analysis was conducted using H&E staining. It was found that CSCs-exo significantly increased the viability, migration, and invasion of bladder cancer cells. High expression of miR-105-5p was observed in CSCs, and treatment with CSCs-exo further increased its expression in bladder cancer cells. GPR12 was identified as a target gene of miR-105-5p and overexpression of GPR12 reversed the effects of miR-105-5p on bladder cancer cell growth and metastasis. Conversely, the anti-tumor effects of miR-105-5p antagomir were observed in xenograft mice. In conclusion, CSCs contribute to the aggressiveness of bladder cancer by transmitting exosomal miR-105-5p to suppress the expression of GPR12. These findings highlight the potential for CSC-targeted therapies in bladder cancer treatment.
Pubmed:
37789353
DOI:
10.1186/s12894-023-01326-2
Lu Y, et al. "Molecular insights into orphan G protein-coupled receptors relevant to ." British journal of pharmacology, 2023.
This study examines the role of G protein-coupled receptors 3, 6, 12, 52, 85, 88, and 139 in the development and progression of schizophrenia. Through an analysis of their expression, signalling mechanisms, and cellular function, in addition to the potential for small molecule development and structural insights, the authors aim to present a comprehensive overview of the current evidence and potential for novel treatments for this mental disorder.
Pubmed:
37605621
DOI:
10.1111/bph.16221
