mProX™ Human GABRA6 Stable Cell Line

Product Information

Target Protein

GABRA6

Target Family

GABAA

Target Protein Species

Human

Host Cell Type

NIH 3T3; CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research

Related Diseases

Childhood Absence Epilepsy; Anxiety

Gene ID

2559

UniProt ID

Q16445

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The primary inhibitory neurotransmitter in the mammalian brain, gabapentin, functions through heteromeric pentameric ligand-gated ion channels, of which GABRA6 is a member of the GABA-A receptor gene family. Many significant pharmacologic drugs, such as ethanol, benzodiazepines, and barbiturates, operate through GABA-A receptors. One possible behavioral correlate of the GABA-A receptor subtype with alpha-6 subunit is cerebellar motor control. The benzodiazepine agonist diazepam can severely impair the postural reflexes of the selectively outbred alcohol-nontolerant (ANT) rat strain. Diazepam-insensitive high-affinity benzodiazepine binding is often absent from ANT cerebella, but in non-selected strains, this binding indicates a granule-cell-specific Alpha-6 subunit-containing GABA-A receptor. The customized GABRA6 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

There are currently no Customer reviews or questions for mProX™ Human GABRA6 Stable Cell Line (S01YF-1123-KX69). Click the button above to contact us or submit your feedback about this product.

FAQ

chat David (Verified Customer)

What assays can the GABRA6 Stable Cell Line be used for? Apr 15 2022

chat Sherry Smith (Creative Biolabs Scientific Support)

This cell line is customized for drug discovery, and it also can be used in ELISA and Western Blots. Apr 15 2022

chat Kathleen (Verified Customer)

Can the GABRA6 stable cell line be used in drug discovery research? Jan 30 2020

chat Sherry Smith (Creative Biolabs Scientific Support)

Yes, the GABRA6 stable cell line can be used in drug discovery research and signaling pathway research. Jan 30 2020

Published Data

Fig.1 Knockdown of REST accelerates the onset of Gabra6 expression and promoter occupancy by NFI.

In CGNPs transduced with lentiviruses expressing scrambled (NS) or REST shrnas and cultivated with Sonic Hedgehog for two days, the REST protein was analyzed Western style.

Ref: Wang, Wei, et al. "Temporal control of a dendritogenesis-linked gene via REST-dependent regulation of nuclear factor I occupancy." Molecular Biology of the Cell 22.6 (2011): 868-879.

Pubmed: 21270437

DOI: 10.1091/mbc.E10-10-0817

Research Highlights

Suicide has been linked to low GABA transmission, and earlier research has linked the GABRA6 rs3219151 T variant to a higher endocrine and physiological stress response.
Gonda, Xenia, et al. "A new stress sensor and risk factor for suicide: the T allele of the functional genetic variant in the GABRA6 gene." Scientific reports 7.1 (2017): 12887.
Pubmed: 29018204 DOI: 10.1038/s41598-017-12776-8

According to this meta-analysis, there is no evidence linking GABRA1 rs2279020 and GABRA6 rs3219151 to the genesis of epilepsy or the response of antiepileptic medications in the Asian and Arabic populations.
Zhang, Tiejun, Yi Yang, and Xiutian Sima. "No association of GABRA1 rs2279020 and GABRA6 rs3219151 polymorphisms with risk of epilepsy and antiepileptic drug responsiveness in Asian and Arabic populations: Evidence from a meta-analysis with trial sequential analysis." Frontiers in Neurology 13 (2022): 996631.
Pubmed: 36188399 DOI: 10.3389/fneur.2022.996631