mProX™ Human GABRA3 Stable Cell Line

Product Information

Target Protein

GABRA3

Target Family

GABAA

Target Protein Species

Human

Host Cell Type

SKBR3; MDA-MB-453; MDA-MB-436; MDA-MB-231; CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research

Related Diseases

Thyrotoxic Periodic Paralysis; Anxiety

Gene ID

2556

UniProt ID

P34903

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The GABRA3 gene in humans codes for the protein known as gamma-aminobutyric acid receptor subunit alpha-3. In the mammalian brain, GABA is the primary inhibitory neurotransmitter that operates through ligand-gated chloride channels called GABAA receptors. Benzodiazepines are among the drugs that can alter the chloride conductance of these channels by binding to the GABAA receptor. It has been determined that GABA-A receptors have at least 16 different subunits. The extracellular ligand binding domains and membrane-integrated ion channel domains make up the five subunits that make up GABA receptors. When ligands attach to these receptors, the channel gets activated. The customized GABRA3 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

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Published Data

Fig.1 Gabra3 is highly expressed in human breast cancer cells

Compared to normal human breast tissues and cells, human breast cancer cells and tissues have elevated levels of Gabra3 expression. Breast cancer survival is adversely linked with high expression of Gabra3. Human breast cancer cell lines SKBR3, MDA-MB-453, MDA-MB-436, MDA-MB-231, and normal human breast epithelial cells HMEL all express the gabra3 protein.

Ref: Gumireddy, Kiranmai, et al. "The mRNA-edited form of GABRA3 suppresses GABRA3-mediated Akt activation and breast cancer metastasis." Nature communications 7.1 (2016): 10715.

Pubmed: 26869349

DOI: 10.1038/ncomms10715

Research Highlights

One important factor influencing the prognosis of breast cancer patients is metastasis. Utilizing data from The Cancer Genome Atlas (TCGA) on breast cancer, 41 genes whose expression is inversely linked with survival were analyzed in order to find molecules involved in the metastatic process.
Gumireddy, Kiranmai, et al. "The mRNA-edited form of GABRA3 suppresses GABRA3-mediated Akt activation and breast cancer metastasis." Nature communications 7.1 (2016): 10715.
Pubmed: 26869349 DOI: 10.1038/ncomms10715

Numerous genes that encode GABAA receptor subunits have been connected to a range of mild to severe epileptic conditions, and it has been demonstrated that the primary relevant pathomechanism is a loss of function.
Niturad, Cristina Elena, et al. "Rare GABRA3 variants are associated with epileptic seizures, encephalopathy and dysmorphic features." Brain 140.11 (2017): 2879-2894.
Pubmed: 29053855 DOI: 10.1093/brain/awx236