mProX™ Human GABRA2 Stable Cell Line

Product Information

Target Protein

GABRA2

Target Family

GABAA

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research; Pain and Addiction Research

Related Diseases

Developmental And Epileptic Encephalopathy; Alcohol Dependence

Gene ID

2555

UniProt ID

P47869

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The GABRA2 gene in humans codes for the protein known as gamma-aminobutyric acid receptor subunit alpha-2. The primary inhibitory neurotransmitter in the mammalian brain, GABA, activates GABA-A receptors, which are ligand-gated chloride channels that include the alpha subunit GABRA2. Benzodiazepines are among the drugs that can alter the chloride conductance of these channels by binding to the GABA-A receptor. Subunit isoforms can be observed throughout development in the brain at different places. The pharmacological and biophysical properties are greatly influenced by the subunit combinations. It has been discovered that GABRA2 mediates anxiolytic action, which is essential for controlling emotions and behavior. The majority of GABRA2 alterations have been connected to teenage behavior and alcoholism. The customized GABRA2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Kathleen

I was very pleased with the QCs of the cell line. Mar 22 2021

chat Verified Customer

chat Patricia

I had a wonderful encounter with this business. Excellent client service was provided. Sep 07 2022

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Repair of the private deletion in B6J is sufficient to restore GABRA2 protein and mRNA levels.

In addition, the original Gabra2KI/KI founder mouse's hippocampal (top panel) and cortical (bottom panel) protein levels are recovered in comparison to B6J and are comparable to those of B6NJ.

Ref: Mulligan, Megan K., et al. "Identification of a functional non-coding variant in the GABA A receptor α2 Subunit of the C57BL/6J mouse reference genome: Major implications for neuroscience research." Frontiers in genetics 10 (2019): 188.

Pubmed: 30984232

DOI: 10.3389/fgene.2019.00188

Research Highlights

In addition, this patient exhibited significant EOEE linked to delayed cognitive development. Since there have only been two cases documented thus far, it is still unclear what the clinical range and functional implications of GABRA2 mutations are.
Maljevic, Snezana, et al. "Novel GABRA2 variants in epileptic encephalopathy and intellectual disability with seizures." Brain 142.5 (2019): e15-e15.
Pubmed: 31032849 DOI: 10.1093/brain/awz079

Genetic variations in GABA receptor subunit alpha-2 (GABRA2) may affect externalizing trajectories by making an individual more vulnerable to parental supervision.
Trucco, Elisa M., et al. "Susceptibility effects of GABA receptor subunit alpha-2 (GABRA2) variants and parental monitoring on externalizing behavior trajectories: Risk and protection conveyed by the minor allele." Development and Psychopathology 28.1 (2016): 15-26.
Pubmed: 25797587 DOI: 10.1017/S0954579415000255