mProX™ Human FPR2 Stable Cell Line

Ren T, et al. "Identification and validation of FPR1, FPR2, IL17RA and TLR7 as immunogenic cell ." Scientific reports, 2023.
The phenomenon of immunogenic cell death (ICD) has garnered significant attention for its therapeutic effectiveness in various diseases. In the same vein, there has been a growing interest in the involvement of immune factors in the pathological process of osteoarthritis (OA). The aim of this study was to use bioinformatics analysis to explore the relationship between ICD-related genes and OA at the genetic level. Using the Limma R package, differentially expressed genes (DEGs) were identified and a weighted gene co-expression network was constructed to determine OA-related module genes. ICD-related genes previously identified were also included in the analysis. Through overlapping the DEGs and ICD-related module genes, hub genes were identified using the Least absolute shrinkage and selection operator and random forest algorithm. The expression levels and diagnostic value of these hub genes were then assessed using Logistic regression. Additionally, the relationship between the hub genes and infiltrating immune cells and immune pathways was investigated using Spearman rank correlation analysis. The expression levels of four hub genes (FPR1, FPR2, IL17RA, and TLR7) were further validated through immunohistochemistry in a rat knee joint model of OA and through qPCR experiment in vitro in IL-1beta induced rat chondrocytes. Ultimately, the knockdown of TLR7 was found to reverse the degradation of collagen II and ADAMTS-5 in IL-1beta-stimulated chondrocytes, solidifying it as an OA biomarker associated with ICD. This study provides potential immune-related biomarkers for the diagnosis of OA and may serve as a reference for monitoring disease treatment.
Pubmed: 37803031   DOI: 10.1038/s41598-023-43440-z

Paterson NM, et al. "Dynamic evolution of bacterial ligand recognition by formyl peptide receptors.." Genome biology and evolution, 2023.
The detection of invasive pathogens is crucial for aiding host immune defense. Cell surface receptors are pivotal in recognizing a variety of microbe-associated molecules, triggering leukocyte recruitment, phagocytosis, release of antimicrobial compounds, and cytokine production. Evolutionary pressures on innate immune receptor genes have contributed to their rapid diversification across plants and animals. However, the functional implications of immune receptor divergence remain unclear. In this study, the authors investigate the loss, diversification, and ligand recognition patterns of formyl peptide receptors (FPRs) in primates and carnivores. Results show that FPR1, essential for innate immune defense in humans, has been lost in New World primates. Amino acid variation in FPR1 and FPR2, consistent with frequent positive selection, was observed in extracellular domains responsible for ligand recognition. Furthermore, assessments of bacterial ligand interactions demonstrate how natural selection may fine-tune FPR activation in response to diverse microbial molecules.
Pubmed: 37776517   DOI: 10.1093/gbe/evad175