mProX™ Human FGR Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1143	Magic™ Human FGR in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

FGR

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

HL-60; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research; Reproductive Research

Related Diseases

Placental Insufficiency and Sarcoma. Among its related pathways are Regulation of actin dynamics for phagocytic cup formation and ADORA2B mediated anti-inflammatory cytokines production

Gene ID

2268

UniProt ID

P09769

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The protein known as Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog, or FGR, is encoded by the FGR gene in humans. This gene belongs to the family of protein tyrosine kinases (PTKs) known as Src. A PTK domain, N-terminal locations for myristoylation and palmitoylation, and SH2 and SH3 domains-which mediate protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively-are all present in the generated protein. The protein is localized to ruffles in the plasma membrane and acts as a negative regulator of adhesion and migration of cells that are stimulated by the signal transduction pathway of beta-2 integrin. When the Epstein-Barr virus infects a person, this gene becomes overexpressed. There are several different splice variants that encode the same protein that have been found. The customized FGR stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Donna

The FGR cell line enable us to develop new insights into its role in disease pathology. Jan 29 2023

chat Verified Customer

chat Jessica

We have found this FGR cell line to be highly reliable and robust, producing consistent results. Sep 24 2022

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Numb binding Vav in HL-60 wt and Fgr KO cells assayed by immunoprecipitation.

For 72 hours, either RA or no treatment (control) was applied to HL-60 wt or Fgr KO cells. Immunoprecipitated numb was then subjected to Western blotting (upper) to detect Vav. It was proven that the immunoprecipitated included numb bait (lower).

Ref: Kazim, Noor, and Andrew Yen. "Role for Fgr and Numb in retinoic acid-induced differentiation and G0 arrest of non-APL AML cells." Oncotarget 12.12 (2021): 1147.

Pubmed: 34136084

DOI: 10.18632/oncotarget.27969

Research Highlights

Umbilical venous flow (QUV) and maternal hemodynamics, such as systemic vascular resistance (SVR) and cardiac output (CO), should be used to better identify fetal growth restriction (FGR) and differentiate it from sickle cell anemia (SGA).
Farsetti, Daniele, et al. "Distinction between SGA and FGR by means of fetal umbilical vein flow and maternal hemodynamics." The Journal of Maternal-Fetal & Neonatal Medicine 35.25 (2022): 6593-6599.
Pubmed: 33938366 DOI: 10.1080/14767058.2021.1918091

These results identify the mitochondrial ROS-Fgr kinase as a critical regulatory axis in diet-induced obesity, insulin resistance, proinflammatory adipose tissue macrophage activation, and hepatic steatosis.
Acín-Pérez, Rebeca, et al. "Fgr kinase is required for proinflammatory macrophage activation during diet-induced obesity." Nature metabolism 2.9 (2020): 974-988.
Pubmed: 32943786 DOI: 10.1038/s42255-020-00273-8