mProX™ Human ESR2 Stable Cell Line

Product Information

Target Protein

ESR2

Target Family

Estrogen Receptor

Target Protein Species

Human

Host Cell Type

FaDu; Cal27; CHO-K1; HEK293

Target Classification

Nuclear Receptor

Target Research Area

Cancer Research; Reproductive Research

Related Diseases

Ovarian Dysgenesis; Thyroid Carcinoma

Gene ID

2100

UniProt ID

Q92731

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

NR3A2, or estrogen receptor beta (ERβ), is one of the two primary forms of estrogen receptors. The ESR2 gene encodes ERβ in humans. ERβ belongs to the nuclear receptor transcription factor superfamily as well as the family of estrogen receptors. The gene product is located in the cytoplasm, mitochondria, and nucleus. It has an N-terminal DNA binding domain and a C-terminal ligand binding domain. The encoded protein forms homo- or hetero-dimers with estrogen receptor α upon binding to 17-β-estradiol, estriol, or analogous ligands. These interactions with specific DNA sequences trigger transcription. The activity of other members of the estrogen receptor family is dominantly inhibited by certain isoforms. Strong tumor suppressor ERβ is involved in many cancer types, including prostate cancer and ovarian cancer. The customized ESR2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Rebecca (Verified Customer)

What is the species for ESR2 cell line? Jul 10 2022

chat Sherry Smith (Creative Biolabs Scientific Support)

We can customize the species you need, such as human, mouse. Jul 10 2022

chat Donna (Verified Customer)

What are other names for ESR2? Jul 24 2023

chat Sherry Smith (Creative Biolabs Scientific Support)

This target may also be called "Est, ERbe, ER[b], Estrb, ERbeta" in publications. Jul 24 2023

Published Data

Fig.1 Regulation of SMR3A expression by ESR2 signaling.

Immunofluorescence labeling b (red signal) showed that fractionated IR promoted basal expression of ESR2 but not ESR1 protein in tumor cell lines, as revealed by Western blot analysis. Hoechst H33342 was used as a counterstain for cell nuclei (blue signal).

Ref: Grünow, Jennifer, et al. "Regulation of submaxillary gland androgen-regulated protein 3A via estrogen receptor 2 in radioresistant head and neck squamous cell carcinoma cells." Journal of Experimental & Clinical Cancer Research 36 (2017): 1-9.

Pubmed: 28166815

DOI: 10.1186/s13046-017-0496-2

Research Highlights

The transcription factor known as estrogen receptor alpha (ERα, encoded by ESR1) is extensively studied and shown to be expressed in over 75% of breast cancers. It is the primary biomarker used to guide endocrine therapy.
Dalal, Hina, et al. "Clinical associations of ESR2 (estrogen receptor beta) expression across thousands of primary breast tumors." Scientific Reports 12.1 (2022): 4696.
Pubmed: 35304506 DOI: 10.1038/s41598-022-08210-3

According to the findings, the absence of ESR2 increased the levels of granulosa and oocyte components, which can help activate mTOR and AKT in ovaries that are Esr2-/-, which in turn increases the activation of primordial follicles.
Chakravarthi, V. Praveen, et al. "A gatekeeping role of ESR2 to maintain the primordial follicle reserve." Endocrinology 161.4 (2020): bqaa037.
Pubmed: 32141511 DOI: 10.1210/endocr/bqaa037