mProX™ Human EPHB4 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1133	Magic™ Human EPHB4 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

EPHB4

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

CNS Research; Reproductive Research

Related Diseases

Capillary Malformation-Arteriovenous Malformation 2 and Lymphatic Malformation 7. Among its related pathways are GPCR Pathway and EPH-Ephrin signaling

Gene ID

2050

UniProt ID

P54760

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human EPHB4 gene encodes the protein known as ephrin type-B receptor 4. Many developmental processes are mediated by ephrin receptors and their ligands, the ephrins, especially in the nervous system. Pherins can be classified into two groups based on their sequence connections and structures: the transmembrane ephrin-B (EFNB) class and the glycosylphosphatidylinositol-anchored ephrin-A (EFNA) class. Based on the similarity of their extracellular domain sequences and their propensity to bind ephrin-A and ephrin-B ligands, the Eph family of receptors is split into two categories. Within the receptor tyrosine kinase (RTK) family, ephrin receptors comprise the biggest subgroup. This gene codes for a protein that interacts to ephrin-B2 and is crucial for the creation of new blood vessels. The customized EPHB4 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Cynthia

Reliable and repeatable throughout the EPHB4 cell line use. Jun 18 2021

chat Verified Customer

chat Timothy

I highly recommend the EPHB4 cell line for anyone working on membrane protein research. Jun 23 2020

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 EphB4 protein expression in human lung cancer cell lines.

Immunoblotting panels of NSCLC and SCLC cell lines to measure the expression of the EphB4 protein. The expression of ephrin-B2 in NSCLC cell lines was also evaluated. The prostate cancer cell line PC3 was included as a positive control, while the immortalized, non-cancerous lung cell line BEAS-2B was employed as a control in this instance.

Ref: Ferguson, Benjamin D., et al. "The EphB4 receptor tyrosine kinase promotes lung cancer growth: a potential novel therapeutic target." PloS one 8.7 (2013): e67668.

Pubmed: 23844053

DOI: 10.1371/journal.pone.0067668

Research Highlights

Chen, Yinnan, Hongmei Zhang, and Yanmin Zhang. "Targeting receptor tyrosine kinase EphB4 in cancer therapy." Seminars in Cancer Biology. VolThis review covers the information on inhibitors of EphB4 kinase activity, examines the structure and function of the EphB4 receptor, and assesses its potential as a target for anticancer therapy. In conclusion, EphB4 represents a difficult yet intriguing cancer treatment target.
Chen, Yinnan, Hongmei Zhang, and Yanmin Zhang. "Targeting receptor tyrosine kinase EphB4 in cancer therapy." Seminars in Cancer Biology. Vol. 56. Academic Press, 2019.. 56. Academic Press, 2019.
Pubmed: 28993206 DOI: 10.1016/j.semcancer.2017.10.002

The ephrin (EFN) ligands of EPH kinases, the biggest family of receptor tyrosine kinases, are also molecules found on the cell surface. This study provides proof that the regulation of blood pressure is influenced by EPHB4 on vascular smooth muscle cells (VSMCs).
Wang, Yujia, et al. "EPHB4 protein expression in vascular smooth muscle cells regulates their contractility, and EPHB4 deletion leads to hypotension in mice." Journal of Biological Chemistry 290.22 (2015): 14235-14244.
Pubmed: 25903126 DOI: 10.1074/jbc.M114.621615