mProX™ Human EPHB3 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1132	Magic™ Human EPHB3 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

EPHB3

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Reproductive Research

Related Diseases

Craniofrontonasal Syndrome. Among its related pathways are GPCR Pathway and EPH-Ephrin signaling

Gene ID

2049

UniProt ID

P54753

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human EPHB3 gene encodes the protein known as ephrin type-B receptor 3. Many developmental processes are mediated by ephrin receptors and their ligands, the ephrins, especially in the nervous system. Pherins can be classified into two groups based on their sequence connections and structures: the transmembrane ephrin-B (EFNB) class and the glycosylphosphatidylinositol-anchored ephrin-A (EFNA) class. Based on the similarity of their extracellular domain sequences and their propensity to bind ephrin-A and ephrin-B ligands, the Eph family of receptors is split into two categories. Within the receptor tyrosine kinase (RTK) family, ephrin receptors comprise the biggest subgroup. This gene codes for a protein that is a receptor for members of the ephrin-B family. The customized EPHB3 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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I think this is a quality EPHB3 cell line for drug discovery. Nov 09 2021

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Very good EPHB3 cell line for drug discovery. Jan 31 2022

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FAQ

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Published Data

Fig.1 EphB3 exhibits ligand-induced inhibition of NSCLC cell migration.

EphB3 overexpression in an ectopic manner in three NSCLC cell lines (H520, H23, and A549, in that order). After three days of puromycin selection, cells were infected with lentiviruses expressing either HA-EphB3 or vector control.

Ref: Li, Guo, et al. "EphB3 suppresses non-small-cell lung cancer metastasis via a PP2A/RACK1/Akt signalling complex." Nature communications 3.1 (2012): 667.

Pubmed: 22314363

DOI: 10.1038/ncomms1675

Research Highlights

Human hepatocellular carcinoma (Eph) receptor tyrosine kinases (RTKs) that produce erythropoietin regulate a number of dynamic cellular processes, such as cell motility, protrusion, proliferation, and cell-fate determination. Eph kinase small-molecule inhibitors are useful instruments for analyzing the physiological and pathological functions of Eph.
Kung, Alvin, et al. "Development of specific, irreversible inhibitors for a receptor tyrosine kinase EphB3." Journal of the American Chemical Society 138.33 (2016): 10554-10560.
Pubmed: 27478969 DOI: 10.1021/jacs.6b05483

These findings suggest that EphB3 may act as a limiter on bone formation and identify it as a potential regulator of osteogenesis, either by itself or in conjunction with other bone-expressed Ephs.
Kamath, Rajay AD, and M. Douglas Benson. "EphB3 as a potential mediator of developmental and reparative osteogenesis." Cells Tissues Organs 212.2 (2023): 125-137.
Pubmed: 34695818 DOI: 10.1159/000520369