mProX™ Human EPHB2 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1131	Magic™ Human EPHB2 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

EPHB2

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

HGC27; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research; Cardiovascular Research

Related Diseases

Bleeding Disorder, Platelet-Type, 22 and Prostate Cancer/Brain Cancer Susceptibility. Among its related pathways are GPCR Pathway and EPH-Ephrin signaling

Gene ID

2048

UniProt ID

P29323

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human EPHB2 gene encodes the protein known as ephrin type-B receptor 2. Many developmental processes are mediated by ephrin receptors and their ligands, the ephrins, especially in the nervous system. Pherins can be classified into two groups based on their sequence connections and structures: the transmembrane ephrin-B (EFNB) class and the glycosylphosphatidylinositol-anchored ephrin-A (EFNA) class. Based on the similarity of their extracellular domain sequences and their propensity to bind ephrin-A and ephrin-B ligands, the Eph family of receptors is split into two categories. Within the receptor tyrosine kinase (RTK) family, ephrin receptors comprise the biggest subgroup. This gene codes for a protein that is a receptor for members of the ephrin-B family. The customized EPHB2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Christopher

We obtained good data in downstream analysis using this EPHB2 cell line. Dec 14 2020

chat Verified Customer

chat Timothy

A good EPHB2 cell line. The company obtains a wide range of membrane protein products which are very reliable. Apr 17 2020

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Validation of EphB2 expression after CRISPR-mediated activation in AGS and HGC27 cell lines.

Western blotting, flow cytometry, and qPCR were used to compare the altered EphB2 expression to the WT control. The EphB2 activator treatment resulted in a significant upregulation of EphB2 expression in both AGS and HGC27 cells, according to the flow cytometric results. The flow cytometry result was validated by the Western blotting.

Ref: Yin, Jie, et al. "EphB2 represents an independent prognostic marker in patients with gastric cancer and promotes tumour cell aggressiveness." Journal of Cancer 11.10 (2020): 2778.

Pubmed: 32226496

DOI: 10.7150/jca.38098

Research Highlights

Based on these findings, EphB2 is a crucial modulator of stress susceptibility and may be a promising target for depression treatment.
Zhang, Ruo-Xi, et al. "EphB2 in the medial prefrontal cortex regulates vulnerability to stress." Neuropsychopharmacology 41.10 (2016): 2541-2556.
Pubmed: 27103064 DOI: 10.1038/npp.2016.58

High EPH/EFN mRNA expression is an independent predictor of poor survival, according to the EPH/EFN cluster analysis. In particular, EPHB2 was associated with poor breast cancer survival in a number of materials, and depending on cell location, EPHB2 protein expression also has prognostic significance.
Husa, Anna-Maria, et al. "EPH/ephrin profile and EPHB2 expression predicts patient survival in breast cancer." Oncotarget 7.16 (2016): 21362.
Pubmed: 26870995 DOI: 10.18632/oncotarget.7246