mProX™ Human EPHB1 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1130	Magic™ Human EPHB1 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

EPHB1

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

U251; U87; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research

Related Diseases

Craniofrontonasal Syndrome and Ovary Serous Adenocarcinoma. Among its related pathways are GPCR Pathway and EPH-Ephrin signaling

Gene ID

2047

UniProt ID

P54762

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human EPHB1 gene encodes the protein known as ephrin type-B receptor 1. Many developmental processes are mediated by ephrin receptors and their ligands, the ephrins, especially in the nervous system. Pherins are classified into two classes: ephrin-A (EFNA) and ephrin-B (EFNB) based on their structures and sequence relationships. Based on the similarity of their extracellular domain sequences and their propensity to bind ephrin-A and ephrin-B ligands, the Eph family of receptors is split into two categories. Within the receptor tyrosine kinase (RTK) family, ephrin receptors comprise the biggest subgroup. This gene codes for a protein that is a receptor for members of the ephrin-B family. The customized EPHB1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Donna

The EPHB1 cell line is an essential tool for anyone studying drug discovery. Feb 15 2022

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chat Laura

The EPHB1 cell line is very good to use and is of high quality and safety. Jan 27 2023

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Cell migration and invasion analysis of glioma cell lines overexpressing EphB1.

Anti-EphB1 antibody was used to immunoprecipitate (IP) extracts of transfected U251 and U87 cells using either the EphB1 vector (EPHB1) or an empty plasmid vector (mock). As said, immunoblotting (IB) was used to probe the immunoprecipitates.

Ref: Teng, Lei, et al. "Ligand-dependent EphB1 signaling suppresses glioma invasion and correlates with patient survival." Neuro-oncology 15.12 (2013): 1710-1720.

Pubmed: 24121831

DOI: 10.1093/neuonc/not128

Research Highlights

This research finds an early help-me signal in neurons that triggers a neuroprotective astrocytic response, which is an appealing therapeutic target but fails in ALS.
Tyzack, Giulia E., et al. "A neuroprotective astrocyte state is induced by neuronal signal EphB1 but fails in ALS models." Nature communications 8.1 (2017): 1164.
Pubmed: 29079839 DOI: 10.1038/s41467-017-01283-z

Repression of EphB1 is linked to a low overall survival rate in juvenile AML patients. EphB1's role in the DDR system suggests that it has a tumor-suppressive role in pediatric AML.
Kampen, K. R., et al. "EphB1 suppression in acute myelogenous leukemia: regulating the DNA damage control system." Molecular Cancer Research 13.6 (2015): 982-992.
Pubmed: 25944917 DOI: 10.1158/1541-7786.MCR-14-0660-T