mProX™ Human EPHA8 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1129	Magic™ Human EPHA8 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

EPHA8

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

SCC-25; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Related Diseases

Among its related pathways are GPCR Pathway and ERK Signaling.

Gene ID

2046

UniProt ID

P29322

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human EPHA8 gene encodes the protein known as ephrin type-A receptor 8. A member of the protein-tyrosine kinase family's ephrin receptor subfamily is encoded by this gene. The extracellular region of receptors in the EPH subfamily usually has two repeats of fibronectin type III and a Cys-rich domain, in addition to a single kinase domain. Based on the similarity of their extracellular domain sequences and their propensity to bind ephrin-A and ephrin-B ligands, the ephrin receptors are classified into two classes. The protein that this gene encodes is involved in short-range contact-mediated axonal guidance throughout the development of the human nervous system. It also serves as a receptor for ephrin A2, A3, and A5. The customized EPHA8 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Carol

The EPHA8 cell line was easy to use, and the support team was responsive to our inquiries. Dec 26 2021

chat Verified Customer

chat Amy

We used the EPHA8 cell line for target validation in our drug development project, and it performed flawlessly. Jan 08 2021

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 High EphA8 expression enhanced the invasion capacity but not proliferation capacity of SCC-25 and H357 cells.

In SCC-25 and H357 cells, the transfection efficiencies of the EphA8 overexpression plasmid or EphA8 siRNA were assessed by measuring the protein levels of EphA8 using western blot analysis.

Ref: Liu, Lijun, Xiaofeng Wang, and Wenzhang Ge. "Epha8 is a prognostic factor for oral tongue squamous cell carcinoma." Medical Science Monitor: International Medical Journal of Experimental and Clinical Research 24 (2018): 7213.

Pubmed: 30300334

DOI: 10.12659/MSM.910909

Research Highlights

In the onset and progression of EOC, EphA8 functions as an oncogene. EphA8 expression detection may be a helpful prognostic indicator, and EphA8 targeting is a unique approach to treating EOC.
Liu, Xiaoqin, et al. "EphA8 is a prognostic marker for epithelial ovarian cancer." Oncotarget 7.15 (2016): 20801.
Pubmed: 26989075 DOI: 10.18632/oncotarget.8018

High expression of EphA8 in GC tissues promotes the growth, invasion, and migration of cancer cells and is a stand-alone risk factor for a poor prognosis in GC. These results imply that EphA8 may provide a novel target for GC diagnosis and/or treatment.
Wang, Yingjing, et al. "EphA8 acts as an oncogene and contributes to poor prognosis in gastric cancer via regulation of ADAM10." Journal of cellular physiology 234.11 (2019): 20408-20419.
Pubmed: 31026069 DOI: 10.1002/jcp.28642