mProX™ Human DDR1 Stable Cell Line

Product Information

Target Protein

DDR1

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

MCF7; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Reproductive Research

Related Diseases

Spondylometaepiphyseal Dysplasia, Short Limb-Hand Type and Pulmonary Fibrosis. Among its related pathways are GPCR Pathway and ERK Signaling

Gene ID

780

UniProt ID

Q08345

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Discoidin domain receptor family, member 1 (DDR1 or CD167a) is a gene found in humans. Receptor tyrosine kinases (RTKs) are essential for cells to communicate with their surroundings. These chemicals play a role in the control of metabolism, differentiation, and cell proliferation. The protein that this gene encodes is an RTK, which is extensively expressed in both normal and mutated epithelial cells and is triggered by different kinds of collagen. This protein is a member of a subfamily of tyrosine kinase receptors that share an extracellular domain homology area with discoidin I, a protein found in Dictyostelium discoideum. All collagens examined thus far have been able to achieve its autophosphorylation. Only epithelial cells, namely those in the kidney, lung, gastrointestinal tract, and brain, have this encoded protein. The customized DDR1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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The performance of the DDR1 cell line was consistent, and the company's support team was responsive and helpful throughout the process. Sep 26 2022

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I have tested the DDR1 cell line from Creative Biolabs as one of the most reliable and versatile options available. Apr 27 2021

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 DDR1 and IGF-IR expression in a panel of cultured cells.

Using polyclonal antibodies against the C-terminus of DDR1 and the C-terminus of IGF-IR, as indicated, a panel of cell lines, including human breast cancer (MCF-7, T47D, ZR-75, MDA-MB-157, MDA-MB-231, and BT-474), human hepatoblastoma (HepG2), and mouse embryo fibroblasts, were subjected to western immunoblot analysis for DDR1 and IGF-IR expression.

Ref: Malaguarnera, Roberta, et al. "Novel cross-talk between IGF-IR and DDR1 regulates IGF-IR trafficking, signaling and biological responses." Oncotarget 6.18 (2015): 16084.

Pubmed: 25840417

DOI: 10.18632/oncotarget.3177

Research Highlights

A collagen-activated receptor tyrosine kinase, discoidin domain receptor 1 (DDR1) is a desirable anti-fibrotic target. Its expression is mostly restricted to epithelial cells found in the skin, kidney, liver, and lungs, among other organs.
Moll, Solange, et al. "DDR1 role in fibrosis and its pharmacological targeting." Biochimica et Biophysica Acta (BBA)-Molecular Cell Research 1866.11 (2019): 118474.
Pubmed: 30954571 DOI: 10.1016/j.bbamcr.2019.04.004

These results pinpoint a mechanism of immune exclusion and propose an immunotherapeutic strategy to enhance immune accessibility by reorganizing the extracellular matrix of the tumor.
Sun, Xiujie, et al. "Tumour DDR1 promotes collagen fibre alignment to instigate immune exclusion." Nature 599.7886 (2021): 673-678.
Pubmed: 34732895 DOI: 10.1038/s41586-021-04057-2